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I have entered into some discussions regarding THC vs THCA analysis for potency testing standards for reporting cannabinoid levels found in medical marijuana. For the next few weeks Montana Biotech will be posting PEER reviewed papers outlining the differing methods for analyzing the cannabinoid contents in cannabis marijuana.
Accuracy, accuracy this is the mantra of a scientist!! We need this accuracy to create valid lines of data to uphold our hypothesis. Current research regarding cannabis and exogenous cannabinoids has been conducted by MANY different labs in MANY different countries!! There has been little if any collaboration on methods for testing cannabinoid contents of cannabis marijuana, hemp.
The discussion was expanded on by Green Jon, senior member, at TreatYourself.com
Here is a qoute:
greeenjon Senior Member
Join Date: Aug 2010
Gas v. Liquid Chromatography
Gas chromatography and liquid chromatography differ in one way that is important to the analysis of cannabinoids. Gas chromatography uses heat and liquid chromatography does not. Heat allows the sample of marijuana to go through a chemical process called decarboxylation, which is a necessary step to determine the exact amount of CBD and THC that can be absorbed into the body. The body will not absorb marijuana that has not been decarboxylated, which is why prior to a patients use marijuana is always exposed to heat. Marijuana is decarboxylated either through smoking, vaporizing, or cooking butter.
Analyzing medical marijuana without decarboxylation, like through liquid chromatography, allows only for the measurement of the maximum THC and CBD possible in a sample. This produces high numbers that are not useful to patients because they will need to decarboxylze marijuana in order to feel its effects.
This is why Gas Chromatography has been the method of choice for the quantification of cannabinoids by both the United Nations Office on Drugs and Crime and the National Institute on Drug Abuse.
Here are two methods outlined by the NIDA (National Institute on Drug Abuse), and the UNODC (United Nations Office on Drugs and Crime): The GC/MS method is good enough for them!!!
GC–MS Analysis of the Total 9-THC Content of Both Drug- and Fiber-Type Cannabis Seeds
Source: Journal of Analytical Toxicology, Volume 24, Number 8, November/December 2000 , pp. 715-717(3)
Publisher: Preston Publications
A GC–MS method was performed to determine the total 9-THC content in both drug- and fiber-type cannabis seeds. Drug-type seeds were found to contain much higher levels of 9-THC (35.6–124 g/g) than fiber (hemp) seeds (0–12 g/g). The majority of 9-THC was found to be located on the surface of the seeds. Approximately 90% of the total 9-THC was removed by a simple, quick wash with chloroform. Washed drug-type seeds contained less than 10 g/g. Separation of the seeds into the kernel and testa showed that the bulk of 9-THC is located in the testa, mainly on the outside. The kernels of drug- and fiber-type cannabis seeds contained less than 2 and 0.5 g 9-THC/g seeds, respectively. Fluctuations in the 9-THC content of different replicates of the same type of seeds could be the result of the degree of contamination on the outside of the seeds.
Affiliations: 1: National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, University, MS 38677 and Departments of Pharmacognosy, School of Pharmacy, University of Mississippi, Uni 2: National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, University, MS 38677 3: ElSohly Laboratories, Incorporated (ELI), 5 Industrial Park Drive, Oxford, MS 38655 4: National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, University, MS 38677 and Department of Pharmaceutics, School of Pharmacy, University of Mississippi, Univ
Publication date: 2000-11-01
Cannabis products are the most widely trafficked drugs worldwide, accounting for 65 per cent of all global seizure cases (1.65 million cases) in 2006. 5,200 metric tons of herb and 1,000 metric tons of resin were seized in 2006. Practically all countries in the world are affected by cannabis trafficking. Similarly, cannabis also remains the most widely used drug worldwide, with an estimated 166 million people having used cannabis in 2006, equivalent to some 4 percent of the global population aged 15-64.
At the same time, especially since the end of the last century, production methods have become increasingly sophisticated, resulting in the availability in illicit markets of a wide range of cannabis products with widely varying levels of the main psychoactive ingredient, delta-9-tetrahydrocannabinol (THC). Most recently, there has lso been a renewed debate about increasing THC content (frequently referred to as “potency”) in illicit cannabis products. All of this requires analytical data which are comparable between laboratories and over time.
However, most countries do not require by law the detailed analysis of the THC content of the different products, and where such analyses are carried out, they use a variety of approaches and experimental designs, reducing the comparability of results. For example, the conversion of natural constituents, such as tetrahydrocannabinolic acid (THCA), by both smoking and under certain analytical conditions into THC, and how this should be reflected in the analytical report, are issues which are not yet standardized worldwide.
On the technological side, the analysis of cannabis products is further complicated by the relatively restricted availability of pure or well defined reference material of THC and other cannabinoids.* The present manual is an updated and significantly revised version of the manual on “Recommended methods for testing cannabis” (ST/NAR/8), which was published in 1987. It has been prepared taking into account both developments in analytical technology and advances in the science of cannabis, and with a view to providing the analytical basis for an objective discussion about changes in THC content over time, and differences between regions and products.
In the end, the choice is YOURS as to who you feel good working with, who can give you the most bang for your buck?! Montana Biotech uses the same methodology as the labs that would be prosecuting you. Montana Biotech will be able to stand with a Hemp farmer in a court of law and state that their cannabis IS hemp! Those labs on the prosecutions side cares not if you have THCA, it is total percent per volume THC that is federally regulated as a schedule 1 narcotic, hemp and hemp product are excluded. The other cannabinoids are not federally regulated when present in volumes higher or lower than the allowable .03% THC/ volume.
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Montana Biotech: UPDATE!
Montana Biotech is proud to introduce an at home THC testing kit to the cannabis consuming public. Grow Buddy™ is a THC testing kit a person could use at home to analyze their own medical marijuana for the comparative presence of cannabinoids! Cannabis is a plant that has been used for thousands of years for its medicinal properties. Now you can test for those cannabinoids at home!
Grow Buddy™ is designed to give maximum results with the minimum of effort. The Grow Buddy™ system is a comparative test made to facilitate the grower, patient, caregiver, or connoisseur to be able to test for cannabinoids at home! Grow Buddy™ was created around a T.L.C. method to help ensure ease of use for ALL ability levels.
The control in this test was ~17% THC by volume… You can tell the size of the dots for Connie Chung (A) and (B) are larger. You can draw you own conclusions!
If you are a patient, you now how hard it is to nail down exactly what strains are best for what, or how to even find the information you need to make those educated decisions. Find a variety/strain YOU like for YOUR condition, test it at home, if it is not already tested, and know what to look for in YOUR medication. Green Crack means very little to a patient who has not used cannabis in their adult lives, but Grow Buddy™ can help that patient know why they like Green Crack for what ails them.
Find/discover varieties or strains that have novel combinations of cannabinoids! Compare relative levels of THC, CBD, CBN, CBC in varieties or strains you know and trust!
Grow Buddy™ will provide an individual the opportunity to select amongst their strains “THE ONE” best suited for further analytical testing by a lab you know and trust. Keep the accuracy of the ever increasing number of potency analysis labs in check! Have an idea of what you have before you spend the TIME and MONEY to get it tested in a lab.
Grow Buddy™ is backed by a potency testing lab with experience in the field of cannabis quality testing. Montana Biotech’s Grow Buddy™ is intended for anyone ready to take their medicine to the next level!
The Grow Buddy™ at home THC testing kit is just $100.00 plus $3.50 for shipping and handling. Order yours today!
Grow Buddy™ is a T.L.C. based method for analyzing cannabis for the presence of THC, CBD, CBN that ANYONE can perform at home! The Test is quick and fun! Ff you have brewed beer, wine, if you consider your self a “good” cook, you can perform a T.L.C. test for THC, CBD, CBN.
SEPARATION AND IDENTIFICATION OF CANNABIS COMPONENTS BY DIFFERENT PLANAR CHROMATOGRAPHY TECHNIQUES (TLC, AMD, OPLC)
N. GALAND*, D. ERNOUF**, F. MONTIGNY***, J. DOLLET* and J. POTHIER*
*UFR des Sciences Pharmaceutiques, Laboratoire de Pharmacognosie, 31 Avenue Monge F-3720 – TOURS
** UFR des Sciences Pharmaceutiques, Laboratoire de Toxicologie, 31 Avenue Monge, F 37200 – TOURS
***UFR des Sciences Pharmaceutiques, Plateau d’Analyses Chimiques et Biochimiques, 31 Avenue Monge,
F-37200 – TOURS
The use of Cannabis is illicit in numerous countries and the increasing consumption has led to a multiplication of scientific studies. New methods of planar chromatography such as Automated Multiple Development (AMD) and Optimum Performance Laminar Chromatography (OPLC) techniques can be used as a substitute for the traditional TLC for the identification and quantification of the Indian hemp components. Each method offers its own advantage: high resolution with neither diffusion nor spot stretching for AMD, speed, efficiency and the possibility of working in semi-preparative mode for OPLC.
Plant DNA Collection Kit™
Montana Biotech is proud to introduce the Plant DNA Collection Kit™. This kit has many functions! The small time patient, to the large scale farmer can gain from this kit / service!
In today’s environment of high competition in the medical marijuana industry, a way to distinguish what you have from your competitors is essential! This kit can be used to create a unique genetic profile of your hybrid! This PCR, gel electrophoresis based test can provide you a “picture” of YOUR plants genetic make-up! With this info in hand, it is easier to obtain a trademark on your variety / strain with the U.S. Patent and Trademark Office.
Another advantage that can be gained with the Plant DNA Collection Kit™ is TIME! TIME from seed, to clone, to harvest! At some point we all need to start from seed, whether it is starting a new variety / strain, or creating your own hybrid. It would be great to be able to weed the males from the females to increase productivity! This can be obtained with male / female sexing! Male / female sexing is a PCR based test. 1-2 weeks after germination of the seed, KNOW if it’s male or female by its GENES. NO GUESS WORK. By time that seedling is ready for cloning, KNOW that it is a FEMALE, and move on!! Maintain the phenotype without having to re-vegetate the plant, our having unwanted clones reducing cost!
Plant DNA Collection Kit™
$49.95 plus $3.50 S&H
Montana Biotech is committed to providing services and products NO OTHER LABORATORY CAN or IS!!
Grow Buddy™ an at Home Cannabinoid Profiling Kit
$100 plus $3.50 S&H
$49.95 plus $3.50 S&H
After over 150 applications for trademarks under the U.S. Patent Office’s proposed “Processed plant matter for medicinal purposes, namely medical marijuana” category, the agency has closed the category due to the, shall we say, ridiculous response. The official reason is because the category was redundant with others, such as business names and manufacturing processes.
Many of the applications for trademarks fall under that former category, such as Colorado’s ReLeaf Center and Total Health Concepts, while some were for specific service names, like Nug Source (an ad company). Foods incorporating marijuana were also a big item for trademark patent, including the now infamous “Dr. Canna Cola.”
The well-known Meyer Keegan from Colorado Springs might take the award for largest number of trademarks and patents applied for by a single individual in the now-defunct medical marijuana category. Keegan applied for trademarks on many terms, such as “ganja,” “Maui Waui,” “Bubblegum,” “Island Sweet Skunk,” and other common strains and smoker terms.
Although the category has been stricken, businesses in the medical marijuana and related fields can still apply for patents and trademarks on their business names, proprietary product names, brands, etc. just as any other business does. The removal of the controversial “medical marijuana” category from the Trademark and Patent Office does not preclude these businesses from operating as would any other business in any other industry.
The so-far undecided patents that were filed with the TPO before closure of the category are still being vetted. Most agree that while it was a nice try, most of Keegan’s attempts at claiming ownership of common names will probably fail. So you won’t have to change your business or product names to avoid a lawsuit for using words like “ganja.”
United States Patent and Trademark Office