Gas v. Liquid Chromatography

Gas v. Liquid Chromatography

Gas chromatography and liquid chromatography differ in one way that is important to the analysis of cannabinoidsGas chromatography uses heat and liquid chromatography does not.  Heat allows the sample of marijuana to go through a chemical process called decarboxylation, which is a necessary step to determine the exact amount of CBD and THC that can be absorbed into the body.  The body will not absorb marijuana that has not been decarboxylated, which is why prior to a patients use marijuana is always exposed to heat.  Marijuana is decarboxylated either through smoking, vaporizing, or cooking butter. Link

Analyzing medical marijuana without decarboxylation, like through liquid chromatography, allows only for the measurement of the maximum THC and CBD possible in a sample.  This produces high numbers that are not useful to patients because they will need to decarboxylze marijuana in order to feel its effects.

This is why Gas Chromatography has been the method of choice for the quantification of cannabinoids by both the United Nations Office on Drugs and Crime (UNODC) and the National Institute on Drug Abuse (NIDA).

!!!Montana Biotech is NOW ACCEPTING PATIENTS!!!

If you have a qualifying medical condition, but cant afford the cost of MMJ registration, we may be able to help.

Wholesale prices are available for all of our products including a 90 day buy back guarantee. Please  feel free to contact us 24/7 with any questions.

Cell: 406-600-6871

NIDA Peer Reviewed Method:

GC–MS Analysis of the Total 9-THC Content of Both Drug- and Fiber-Type Cannabis Seeds

Authors: Ross S.A.1; Mehmedic Z.2; Murphy T.P.3; ElSohly M.A.4

Source: Journal of Analytical Toxicology, Volume 24, Number 8, November/December 2000 , pp. 715-717(3)

Publisher: Preston Publications

Abstract:

A GC–MS method was performed to determine the total 9-THC content in both drug– and fiber-type cannabis seeds. Drug-type seeds were found to contain much higher levels of 9-THC (35.6–124 g/g) than fiber (hemp) seeds (0–12 g/g). The majority of 9-THC was found to be located on the surface of the seeds. Approximately 90% of the total 9-THC was removed by a simple, quick wash with chloroform. Washed drug-type seeds contained less than 10 g/g. Separation of the seeds into the kernel and testa showed that the bulk of 9-THC is located in the testa, mainly on the outside. The kernels of drug- and fiber-type cannabis seeds contained less than 2 and 0.5 g 9-THC/g seeds, respectively. Fluctuations in the 9-THC content of different replicates of the same type of seeds could be the result of the degree of contamination on the outside of the seeds.

Language: English

Document Type:

Affiliations: 1: National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, University, MS 38677 and Departments of Pharmacognosy, School of Pharmacy, University of Mississippi, Uni 2: National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, University, MS 38677 3: ElSohly Laboratories, Incorporated (ELI), 5 Industrial Park Drive, Oxford, MS 38655 4: National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, University, MS 38677 and Department of Pharmaceutics, School of Pharmacy, University of Mississippi, Univ

Publication date: 2000-11-01

UNODC Peer Reviewed Method:

UN methods for THC testing

1. Introduction
1.1 Background

Cannabis products are the most widely trafficked drugs worldwide, accounting for 65 per cent of all global seizure cases (1.65 million cases) in 2006. 5,200 metric tons of herb and 1,000 metric tons of resin were seized in 2006. Practically all countries in the world are affected by cannabis trafficking. Similarly, cannabis also remains the most widely used drug worldwide, with an estimated 166 million people having used cannabis in 2006, equivalent to some 4 percent of the global population aged 15-64.

At the same time, especially since the end of the last century, production methods have become increasingly sophisticated, resulting in the availability in illicit markets of a wide range of cannabis products with widely varying levels of the main psychoactive ingredient, delta-9-tetrahydrocannabinol (THC). Most recently, there has lso been a renewed debate about increasing THC content (frequently referred to as “potency”) in illicit cannabis products. All of this requires analytical data which are comparable between laboratories and over time.

However, most countries do not require by law the detailed analysis of the THC content of the different products, and where such analyses are carried out, they use a variety of approaches and experimental designs, reducing the comparability of results. For example, the conversion of natural constituents, such as tetrahydrocannabinolic acid (THCA), by both smoking and under certain analytical conditions into THC, and how this should be reflected in the analytical report, are issues which are not yet standardized worldwide.

On the technological side, the analysis of cannabis products is further complicated by the relatively restricted availability of pure or well defined reference material of THC and other cannabinoids.* The present manual is an updated and significantly revised version of the manual on “Recommended methods for testing cannabis” (ST/NAR/8), which was published in 1987. It has been prepared taking into account both developments in analytical technology and advances in the science of cannabis, and with a view to providing the analytical basis for an objective discussion about changes in THC content over time, and differences between regions and products.

Cannabis TV Documentaries:

Hemp for Victory

Reefer Madness

The BEST Cannabis / Marijuana Propaganda Films / Movies of ALL TIME!

Marijuana: A Chronic History Presented by the History Channel

NGC’s Drugs Inc.: Marijuana

NGC’s Marijuana Nation

PBS’ Botany of Desire

CNBC’s Marijuana Inc.

TEA Party Eat your Heart Out!! The Bundy’s Were Here 1st!!

Links:

Peer Reviewed Research

MMJ Clinics / Doctors Recommendations

MMJ Dispensaries/Caregivers: Find Them Here

MMJ Strains / Varieties: Find Them Here Potency Tested

Ed Rosenthal Runs For President in 2012? Stephen’s Colbert SuperPac Funding?

Fastest way to determine cannabis seedling sex!

Grow Buddy Thin Layer Chromatography THC, CBD test kit

Grow Buddy Instructional Video

Grow Buddy Replacement T.L.C. Supplies

Plant DNA Collection Kit

Montana Biotech Products and Services

Cannabinoid Facts

Specialist Want Regulations / Oversight of Medical Marijuana Analysis Labs, Montana Biotech Embraces Open Dialog

Cannalytics™ Cannabinoid Fingerprinting Test by Alpha Nova vs Grow Buddy™ at Home THC, CBD, CBN Test Kit by Montana Biotech Comparing Two Competing Thin Layer Chromatography T.L.C Methods

QUALTITATIVE vs. QAUNTITATIVE Analysis of Cannabis THC, CBD, CBN Cannabinoid Levels

Ed Rosenthal and the Party-Party’s Platform for America 2012!

A Little Ed History and Education! Wiki Style!

Peer Reviewed Research:

ADD /ADHD, Medical Marijuana and its potential Treatment.

Addiction Risk-Physical of using Medical Marijuana.

Alcoholism and Medical Marijuana.

ALS, Lou Gehrig’s Disease Medical Cannabis Therapy

Cannabis Marijuana Use and AMOTIVATIONAL SYNDROME

Alzheimer’s Disease Progression Prevention Cannabinoids

Medical Cannabis Marijuana and ANTI-INFLAMMATORY PROPERTIES of Cannabinoids

ANTI-BACTERIAL PROPERTIES of Cannabinoids, Cannabidiol,  Cannabichromene, Cannabigerol, Δ9-tetrahydrocannabinol, Cannabinol

Medical Cannabis Marijuana and ANTI-INFLAMMATORY PROPERTIES of Cannabinoids

Cannabinoids, Cannabidiol CBD,  Cannabichromene CBC, Cannabigerol CBG, Δ9-tetrahydrocannabinol THC, Cannabinol CBN and Cannabis Marijuana Antioxidant Properties

Cannabis Marijuana and ANXIOLYTIC, Also Known as Antipanic or Antianxiety Agents, EFFECTS of CBD Cannabidiol for Treatment of Anxiety

Call Montana Biotech today for more information on setting up a Grow Room, Help or a consultation!

Cell: 406-600-6871

E-Mail: MontanaBiotech@gmail.com

Twitter: @MontananBiotech @GreeneAcresCare @GrowCannabis

Facebook: http://www.facebook.com/joseph.lacerenza

Donate to Cannabis Research at Montana Biotech

Cannalytics™ Cannabinoid Finger Printing Test Kit

Cannalytics™ Cannabinoid Fingerprinting Test Kit

“…It doesn’t really work…”

“…And yes, without a very accurate scale your results…”

 

 

!!!Montana Biotech is NOW ACCEPTING PATIENTS!!!

If you have a qualifying medical condition, but cant afford the cost of MMJ registration, we may be able to help.

Wholesale prices are available for all of our products including a 90 day buy back guarantee. Please  feel free to contact us 24/7 with any questions.

Cell: 406-600-6871

 “…This is just a knock off of the cannanlyse thc testing kit but with out a lot of supplies that come with the cannalyse kit ,also even though TLC is a common way of testing elements the dye and extraction fluids must be correct and the directions must be exact that come with the kits. How would you get results were is the reference chart and why do you need to chemicals for extraction and developing. This looks like a older kit that was put out on the market years ago in the Netherlands…”

To start off, if this company Alpha Nova operated in the U.S., I WOULD SUE THEM! Their claim of being able to use a PREDETERMINED chart to quantify cannabinoids is FALSE, better yet an out right, bold face LIE! Cannalytics is MARKETED as a quantitative test, and TLC is NOT a quantitative analysis method!

Montana Biotech, at least, offers THC, CBD, CBN cannabinoid standards!

According to the UNODC

“…Unlike the comparison of two samples in order to assess whether they are the same (or different), when quantitative analysis is essential, assessment of origin is qualitative, since the sample under study and the reference samples are, in fact, different…”

Cannalytics™ Cannabinoid Fingerprinting Test by Alpha Nova:

20-25 Tests $199 plus $19.99 S/H

Contents for the 20/25 test kit:

5 TLC plates for 4/5 samples per plate
1 Container of coloring dye powder
1 Bottle of test fluid
1 Developing container/jar
25 Small plastic reaction tubes
1 Reaction tube rack
25 Capillary tubes
1 Capillary tube extraction bulb
1 Glass pipette assembly – 1ml
1 Spray bottle
1 Jet flame butane torch lighter
5 Pairs protective gloves
1 User’s manual

PRICE PER SAMPLE TESTED:

$199 Kit + $19.99 S/H = $218.99

$218.99/ 20-25 Samples = $10.95 – $8.75

Grow Buddy™ at Home THC, CBD, CBN Test Kit by Montana Biotech

16-20 Tests $100 plus $3.50 S/H

Contents for the 16/20 test kit:

4 TLC plates for 4/5 samples per plate
1 Container of coloring dye powder
1 Bottle of test fluid
1 Developing container/jar
20 Small plastic reaction tubes
1 Reaction tube rack
10 Capillary tubes
1 Capillary tube extraction bulb
1 Glass pipette assembly – 1 microliter
1 Spray bottle
1 Pair protective gloves
1 User’s manual

PRICE PER SAMPLE TESTED:

$100 Kit + $3.50 S/H = $103.50

$103.50/ 16-20 Samples = $6.45 – $5.17

SIRCHIE

Thin Layer Chromatography of Marijuana Kit with 6 Tests

This is a rapid, simplified, specific test for marijuana utilizing a laboratory technique that any officer may perform. No false positives will be encountered. It has a sensitivity of 120 times that of the Duquenois test. Special consideration can be given samples previously thought of as insufficient for analysis. A complete kit for performing thin layer chromatography that permits six complete, individual tests is available.

MZ020 Kit Contents:

  • 6 – MZ020A Thin Layer Chromatography Test
  • 1 – MZ010B Extract Reagent #1, 1 Oz
  • 1 – KCP228A Glass Jar with Closure, 4 Oz, Labeled Mobile Phase
  • 1 – KCP228A Glass Jar with Closure, 4 Oz, Labeled Color Developing
  • 1 – 288DP Disposable Pipettes with Capillary and 3ml Bulb
  • 1 – EIL011 Evidence Identification Labels, “Crime Evidence”
  • 1 – KCP321 Photo: Chromatography of Marijuana Comparison
  • 1 – KCP1391 Plastic Tweezers, 4.75″
  • 1 – KCP165 Glass Stirring Rod, 5″
  • 1 – KCP154 Pipette with Suction Bulb
  • 1 – MZ020C Texturized, Molded-Plastic Carrying Case, 14.25″ x 8.25″ x 7″

6 tests for $81.95 plus S/H

This kit is marketed to police officers! There is NO mention of a THC “chart” to help quantify the cannabis sample. You would think the cops would have a good kit!

http://store.sirchie.com/Thin-Layer-Chromatography-of-Marijuana-Kit-with-6-Tests-P1468.aspx

Introduction: Grow Buddy™ at Home THC, CBD, CBN, Thin Layer Chromatography, TLC , Test Kit

Grow Buddy Thin Layer Chromatography THC, CBD test kit

Grow Buddy Instructional Video

Grow Buddy Replacement T.L.C. Supplies

Plant DNA Collection Kit

Montana Biotech Products and Services

Web: https://montanabiotech.wordpress.com/
Facebook: http://facebook.com/joseph.lacerenza

Twitter: @MontananBiotech, @GreeneAcresCares, @GrowCannabis

Step 1: Grow Buddy™ at Home THC, CBD, CBN, Thin Layer Chromatography, TLC , Test Kit

Step 2: Grow Buddy™ at Home THC, CBD, CBN, Thin Layer Chromatography, TLC , Test Kit

Step 3: Grow Buddy™ at Home THC, CBD, CBN, Thin Layer Chromatography, TLC , Test Kit

Step 4: Grow Buddy™ at Home THC, CBD, CBN, Thin Layer Chromatography, TLC , Test Kit

Step 5: Grow Buddy™ at Home THC, CBD, CBN, Thin Layer Chromatography, TLC , Test Kit

Step 6: Grow Buddy™ at Home THC, CBD, CBN, Thin Layer Chromatography, TLC , Test Kit

Step 7: Grow Buddy™ at Home THC, CBD, CBN, Thin Layer Chromatography, TLC , Test Kit

Step 8: Grow Buddy™ at Home THC, CBD, CBN, Thin Layer Chromatography, TLC , Test Kit

Step 9: Grow Buddy™ at Home THC, CBD, CBN, Thin Layer Chromatography, TLC , Test Kit

Grow Buddy™ is a T.L.C. based method for analyzing cannabis for the presence of THC, CBD, CBN that ANYONE can perform at home! The Test is quick and fun! if you have brewed beer, wine, if you consider your self a “good” cook, you can perform a T.L.C. test for THC, CBD, CBN.


TLC, UNODC (United Nations Office on Drug and Crime)

TLC was the method of choice for this study as it provides a means of rapidly and visually analysing a number of samples simultaneously. Unlike the comparison of two samples in order to assess whether they are the same (or different), when quantitative analysis is essential, assessment of origin is qualitative, since the sample under study and the reference samples are, in fact, different. There is thus little point in using more powerful techniques such as high-performance liquid chromatography as the quantitative information provided is likely to confuse the picture. The TLC system was designed to give information on the cannabivarins in addition to the more commonly studied cannabinoids. The principal cannabinoids observed using this system are given in table 2. Sample preparation and detection were as previously described [ 47] [ 48] . The solvent system used was a mixture of chloroform (ethanol-free) and l,l-dichloroethane (15:10) with simple ascending TLC on 10 x 20 cm silica gel pre-coated plates with a layer thickness of 0.25 mm (E. Merck, Darmstadt, G.F.R., Art. No. 5729) [ 46] . Each extract was analysed within one hour of preparation. Twenty different samples of herbal cannabis and cannabis resin from several different countries were subjected to hot-solvent extraction to simulate the illicit preparation of liquid cannabis. The resulting chromatograms were compared with those of the starting material..

The control in this test was ~17% THC by volume… You can tell the size of the dots for Connie Chung (A) and (B) are larger. You can draw you own conclusions!

Connie Chung (A) THC Levels

Connie Chung (B) THC Levels

SEPARATION AND IDENTIFICATION OF CANNABIS COMPONENTS BY DIFFERENT PLANAR CHROMATOGRAPHY TECHNIQUES (TLC, AMD, OPLC)

N. GALAND*, D. ERNOUF**, F. MONTIGNY***, J. DOLLET* and J. POTHIER*
*UFR des Sciences Pharmaceutiques, Laboratoire de Pharmacognosie, 31 Avenue Monge F-3720 – TOURS
** UFR des Sciences Pharmaceutiques, Laboratoire de Toxicologie, 31 Avenue Monge, F 37200 – TOURS
***UFR des Sciences Pharmaceutiques, Plateau d’Analyses Chimiques et Biochimiques, 31 Avenue Monge,
F-37200 – TOURS

Abstract
The use of Cannabis is illicit in numerous countries and the increasing consumption has led to a multiplication of scientific studies. New methods of planar chromatography such as Automated Multiple Development (AMD) and Optimum Performance Laminar Chromatography (OPLC) techniques can be used as a substitute for the traditional TLC for the identification and quantification of the Indian hemp components. Each method offers its own advantage: high resolution with neither diffusion nor spot stretching for AMD, speed, efficiency and the possibility of working in semi-preparative mode for OPLC.

Cannabis_components_by_TLC_OPLC_AMD

!!SALE for September!! Grow Buddy Thin Layer Chromatography THC, CBD, Cannabis Cannabinoids Test Kit !!SALE for September!!

The Grow Buddy™ at home THC testing kit is just $100.00 plus $3.50 for shipping and handling in the U.S. Order yours today!

The Grow Buddy™ at home THC testing kit is just $100.00 plus $50.00 for International Orders, shipping and handling. Order yours today!

Cannabis TV Documentaries:

Hemp for Victory

Reefer Madness

The BEST Cannabis / Marijuana Propaganda Films / Movies of ALL TIME!

Marijuana: A Chronic History Presented by the History Channel

NGC’s Drugs Inc.: Marijuana

NGC’s Marijuana Nation

PBS’ Botany of Desire

CNBC’s Marijuana Inc.

TEA Party Eat your Heart Out!! The Bundy’s Were Here 1st!!

Links:

Peer Reviewed Research

MMJ Clinics / Doctors Recommendations

MMJ Dispensaries/Caregivers: Find Them Here

MMJ Strains / Varieties: Find Them Here Potency Tested

Ed Rosenthal Runs For President in 2012? Stephen’s Colbert SuperPac Funding?

Fastest way to determine cannabis seedling sex!

Grow Buddy Thin Layer Chromatography THC, CBD test kit

Grow Buddy Instructional Video

Grow Buddy Replacement T.L.C. Supplies

Plant DNA Collection Kit

Montana Biotech Products and Services

Cannabinoid Facts

Specialist Want Regulations / Oversight of Medical Marijuana Analysis Labs, Montana Biotech Embraces Open Dialog

Cannalytics™ Cannabinoid Fingerprinting Test by Alpha Nova vs Grow Buddy™ at Home THC, CBD, CBN Test Kit by Montana Biotech Comparing Two Competing Thin Layer Chromatography T.L.C Methods

QUALTITATIVE vs. QAUNTITATIVE Analysis of Cannabis THC, CBD, CBN Cannabinoid Levels

Ed Rosenthal and the Party-Party’s Platform for America 2012!

A Little Ed History and Education! Wiki Style!

Peer Reviewed Research:

ADD /ADHD, Medical Marijuana and its potential Treatment.

Addiction Risk-Physical of using Medical Marijuana.

Alcoholism and Medical Marijuana.

ALS, Lou Gehrig’s Disease Medical Cannabis Therapy

Cannabis Marijuana Use and AMOTIVATIONAL SYNDROME

Alzheimer’s Disease Progression Prevention Cannabinoids

Medical Cannabis Marijuana and ANTI-INFLAMMATORY PROPERTIES of Cannabinoids

ANTI-BACTERIAL PROPERTIES of Cannabinoids, Cannabidiol,  Cannabichromene, Cannabigerol, Δ9-tetrahydrocannabinol, Cannabinol

Medical Cannabis Marijuana and ANTI-INFLAMMATORY PROPERTIES of Cannabinoids

Cannabinoids, Cannabidiol CBD,  Cannabichromene CBC, Cannabigerol CBG, Δ9-tetrahydrocannabinol THC, Cannabinol CBN and Cannabis Marijuana Antioxidant Properties

Cannabis Marijuana and ANXIOLYTIC, Also Known as Antipanic or Antianxiety Agents, EFFECTS of CBD Cannabidiol for Treatment of Anxiety

Call Montana Biotech today for more information on setting up a Grow Room, Help or a consultation!

Cell: 406-600-6871

E-Mail: MontanaBiotech@gmail.com

Twitter: @MontananBiotech @GreeneAcresCare @GrowCannabis

Facebook: http://www.facebook.com/joseph.lacerenza

Donate to Cannabis Research at Montana Biotech

Cannalytics™ Cannabinoid Fingerprinting Test by Alpha Nova vs Grow Buddy™ at Home THC, CBD, CBN Test Kit by Montana Biotech Comparing Two Competing Thin Layer Chromatography T.L.C Methods

Cannalytics™ Cannabinoid Fingerprinting Test by Alpha Nova vs Grow Buddy™ at Home THC, CBD, CBN Test Kit by Montana Biotech Comparing Two Competing Thin Layer Chromatography T.L.C MethodsUPDATE: Grow Buddy Replacement Thin Layer Chromatography TLC Parts, Pieces, and Accessories, from Montana Biotech

!!!Montana Biotech is NOW ACCEPTING PATIENTS!!!

If you have a qualifying medical condition, but cant afford the cost of MMJ registration, we may be able to help.

Wholesale prices are available for all of our products including a 90 day buy back guarantee. Please  feel free to contact us 24/7 with any questions.

Cell: 406-600-6871

I received a comment on my blog recently mentioning the comparison of Alpha Nova’s Cannalytics™ cannabinoid thin layer chromatography testing system and Montana Biotech’s Grow Buddy™ T.L.C. test kit. Thin layer chromatography is a method for testing different compounds using a solvent and glass plates coated with silica gel and a dye for visualizing the results. The method is OLD, and nobody has the patent on it! According to the UNODC, T.L.C is not used for quantification, but THC,CBD, CBN, qualification. The thin layer chromatography method must be used with a standard to be quantitative in any capacity.

Cannalytics™  Grow Buddy™ 
by Alpha Nova by Montana Biotech
Equiptment:
THC, CBD, CBN No Yes
 Standards
Technical support No Yes
Replacement Parts No Yes
T.L.C. Plates Yes Yes
Extraction Solvent Yes Yes
Developing Solution Yes Yes
T.L.C. Dye Yes Yes
T.L.C. Dye Sprayer Yes Yes
Gloves Yes Yes
1.5mL tubes Yes Yes
Capillary tubes Yes Yes
Micro Pipette Yes Yes
Large Pipettes Yes Yes
Developing Yes Yes
chamber
Instructions Yes Yes

“…Unlike the comparison of two samples in order to assess whether they are the same (or different), when quantitative analysis is essential, assessment of origin is qualitative, since the sample under study and the reference samples are, in fact, different…”

So, the argument is mute, T.L.C. is NOT a quantitative testing method according to the UNODC!  You would think a European company would KNOW that!! In the end, Cannalytics™ Cannabinoid Fingerprinting Test by Alpha Nova vs Grow Buddy™ at Home THC, CBD, CBN Test Kit by Montana Biotech, is NO comparison! A chart for quantifying cannabinoid levels is a JOKE!!

Cannalytics™ Cannabinoid Fingerprinting "THC" chart

THIS is the Cannalytics™ Cannabinoid Fingerprinting “THC” chart. Again, what a JOKE, an internal THC, CBD, CBN standard MUST be ran with each T.L.C. plate to be valid.

Cannalytics™ Cannabinoid Fingerprinting Test by Alpha Nova:

20-25 Tests $199 plus $19.99 S/H

Contents for the 20/25 test kit:

5 TLC plates for 4/5 samples per plate
1 Container of coloring dye powder
1 Bottle of test fluid
1 Developing container/jar
25 Small plastic reaction tubes
1 Reaction tube rack
25 Capillary tubes
1 Capillary tube extraction bulb
1 Glass pipette assembly – 1ml
1 Spray bottle
1 Jet flame butane torch lighter
5 Pairs protective gloves
1 User’s manual

PRICE PER SAMPLE TESTED:

$199 Kit + $19.99 S/H = $218.99

$218.99/ 20-25 Samples = $10.95 – $8.75

Grow Buddy™ at Home THC, CBD, CBN Test Kit by Montana Biotech

16-20 Tests $100 plus $3.50 S/H

Contents for the 16/20 test kit:

4 TLC plates for 4/5 samples per plate
1 Container of coloring dye powder
1 Bottle of test fluid
1 Developing container/jar
20 Small plastic reaction tubes
1 Reaction tube rack
10 Capillary tubes
1 Capillary tube extraction bulb
1 Glass pipette assembly – 1 microliter
1 Spray bottle
1 Pair protective gloves
1 User’s manual

PRICE PER SAMPLE TESTED:

$100 Kit + $3.50 S/H = $103.50

$103.50/ 16-20 Samples = $6.45 – $5.17

Links:

Cannabinoid Facts

Grow Buddy™ T.L.C. test kit

Specialist Want Regulations / Oversight of Medical Marijuana Analysis Labs, Montana Biotech Embraces Open Dialog

THC, CBD, CBN, Cannabinoid Standards  for the Grow Buddy™, COMING SOON!!!

Introduction: Grow Buddy™ at Home THC, CBD, CBN, Thin Layer Chromatography, TLC , Test Kit

Step 1: Grow Buddy™ at Home THC, CBD, CBN, Thin Layer Chromatography, TLC , Test Kit

Step 2: Grow Buddy™ at Home THC, CBD, CBN, Thin Layer Chromatography, TLC , Test Kit

Step 3: Grow Buddy™ at Home THC, CBD, CBN, Thin Layer Chromatography, TLC , Test Kit

Step 4: Grow Buddy™ at Home THC, CBD, CBN, Thin Layer Chromatography, TLC , Test Kit

Step 5: Grow Buddy™ at Home THC, CBD, CBN, Thin Layer Chromatography, TLC , Test Kit

Step 6: Grow Buddy™ at Home THC, CBD, CBN, Thin Layer Chromatography, TLC , Test Kit

Step 7: Grow Buddy™ at Home THC, CBD, CBN, Thin Layer Chromatography, TLC , Test Kit

Step 8: Grow Buddy™ at Home THC, CBD, CBN, Thin Layer Chromatography, TLC , Test Kit

Step 9: Grow Buddy™ at Home THC, CBD, CBN, Thin Layer Chromatography, TLC , Test Kit

THC, CBD, CBN, Cannabinoid Standards  for the Grow Buddy™, COMING SOON!!!

Grow Buddy™ is a T.L.C. based method for analyzing cannabis for the presence of THC, CBD, CBN that ANYONE can perform at home! The Test is quick and fun! if you have brewed beer, wine, if you consider your self a “good” cook, you can perform a T.L.C. test for THC, CBD, CBN.

THC, CBD, CBN, Cannabinoid Standards  for the Grow Buddy™, COMING SOON!!!


TLC, UNODC (United Nations Office on Drug and Crime)

TLC was the method of choice for this study as it provides a means of rapidly and visually analysing a number of samples simultaneously. Unlike the comparison of two samples in order to assess whether they are the same (or different), when quantitative analysis is essential, assessment of origin is qualitative, since the sample under study and the reference samples are, in fact, different. There is thus little point in using more powerful techniques such as high-performance liquid chromatography as the quantitative information provided is likely to confuse the picture. The TLC system was designed to give information on the cannabivarins in addition to the more commonly studied cannabinoids. The principal cannabinoids observed using this system are given in table 2. Sample preparation and detection were as previously described [ 47] [ 48] . The solvent system used was a mixture of chloroform (ethanol-free) and l,l-dichloroethane (15:10) with simple ascending TLC on 10 x 20 cm silica gel pre-coated plates with a layer thickness of 0.25 mm (E. Merck, Darmstadt, G.F.R., Art. No. 5729) [ 46] . Each extract was analysed within one hour of preparation. Twenty different samples of herbal cannabis and cannabis resin from several different countries were subjected to hot-solvent extraction to simulate the illicit preparation of liquid cannabis. The resulting chromatograms were compared with those of the starting material..

The control in this test was ~17% THC by volume… You can tell the size of the dots for Connie Chung (A) and (B) are larger. You can draw you own conclusions!

Connie Chung (A) THC Levels

Connie Chung (B) THC Levels

THC, CBD, CBN, Cannabinoid Standards  for the Grow Buddy™, COMING SOON!!!

SEPARATION AND IDENTIFICATION OF CANNABIS COMPONENTS BY DIFFERENT PLANAR CHROMATOGRAPHY TECHNIQUES (TLC, AMD, OPLC)

N. GALAND*, D. ERNOUF**, F. MONTIGNY***, J. DOLLET* and J. POTHIER*
*UFR des Sciences Pharmaceutiques, Laboratoire de Pharmacognosie, 31 Avenue Monge F-3720 – TOURS
** UFR des Sciences Pharmaceutiques, Laboratoire de Toxicologie, 31 Avenue Monge, F 37200 – TOURS
***UFR des Sciences Pharmaceutiques, Plateau d’Analyses Chimiques et Biochimiques, 31 Avenue Monge,
F-37200 – TOURS

Abstract
The use of Cannabis is illicit in numerous countries and the increasing consumption has led to a multiplication of scientific studies. New methods of planar chromatography such as Automated Multiple Development (AMD) and Optimum Performance Laminar Chromatography (OPLC) techniques can be used as a substitute for the traditional TLC for the identification and quantification of the Indian hemp components. Each method offers its own advantage: high resolution with neither diffusion nor spot stretching for AMD, speed, efficiency and the possibility of working in semi-preparative mode for OPLC.

Cannabis_components_by_TLC_OPLC_AMD

The Grow Buddy™ at home THC testing kit is just $100.00 plus $3.50 for shipping and handling in the U.S. Order yours today!

The Grow Buddy™ at home THC testing kit is just $100.00 plus $50.00 for International Orders, shipping and handling. Order yours today! Grow Buddy T.L.C. Cannabinoids THC, CBD  Test Kit Instructions Dutch Translation

Cannabis TV Documentaries:

Hemp for Victory

Reefer Madness

The BEST Cannabis / Marijuana Propaganda Films / Movies of ALL TIME!

Marijuana: A Chronic History Presented by the History Channel

NGC’s Drugs Inc.: Marijuana

NGC’s Marijuana Nation

PBS’ Botany of Desire

CNBC’s Marijuana Inc.

TEA Party Eat your Heart Out!! The Bundy’s Were Here 1st!!

Links:

Peer Reviewed Research

MMJ Clinics / Doctors Recommendations

MMJ Dispensaries/Caregivers: Find Them Here

MMJ Strains / Varieties: Find Them Here Potency Tested

Ed Rosenthal Runs For President in 2012? Stephen’s Colbert SuperPac Funding?

Fastest way to determine cannabis seedling sex!

Grow Buddy Thin Layer Chromatography THC, CBD test kit

Grow Buddy Instructional Video

Grow Buddy Replacement T.L.C. Supplies

Plant DNA Collection Kit

Montana Biotech Products and Services

Cannabinoid Facts

Specialist Want Regulations / Oversight of Medical Marijuana Analysis Labs, Montana Biotech Embraces Open Dialog

Cannalytics™ Cannabinoid Fingerprinting Test by Alpha Nova vs Grow Buddy™ at Home THC, CBD, CBN Test Kit by Montana Biotech Comparing Two Competing Thin Layer Chromatography T.L.C Methods

QUALTITATIVE vs. QAUNTITATIVE Analysis of Cannabis THC, CBD, CBN Cannabinoid Levels

Ed Rosenthal and the Party-Party’s Platform for America 2012!

A Little Ed History and Education! Wiki Style!

Peer Reviewed Research:

ADD /ADHD, Medical Marijuana and its potential Treatment.

Addiction Risk-Physical of using Medical Marijuana.

Alcoholism and Medical Marijuana.

ALS, Lou Gehrig’s Disease Medical Cannabis Therapy

Cannabis Marijuana Use and AMOTIVATIONAL SYNDROME

Alzheimer’s Disease Progression Prevention Cannabinoids

Medical Cannabis Marijuana and ANTI-INFLAMMATORY PROPERTIES of Cannabinoids

ANTI-BACTERIAL PROPERTIES of Cannabinoids, Cannabidiol,  Cannabichromene, Cannabigerol, Δ9-tetrahydrocannabinol, Cannabinol

Medical Cannabis Marijuana and ANTI-INFLAMMATORY PROPERTIES of Cannabinoids

Cannabinoids, Cannabidiol CBD,  Cannabichromene CBC, Cannabigerol CBG, Δ9-tetrahydrocannabinol THC, Cannabinol CBN and Cannabis Marijuana Antioxidant Properties

Cannabis Marijuana and ANXIOLYTIC, Also Known as Antipanic or Antianxiety Agents, EFFECTS of CBD Cannabidiol for Treatment of Anxiety

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