Grow Buddy THC CBD Home Test Kit Instructions English
1.) Remove all items from the area not needed for your testing.
2.) Collect materials not included in the kit needed for testing.
b) A scale accurate to .1g
c) Timer, or some way to keep track of elapsed time
d) Some way to capture an image of the T.L.C. plates after being sprayed with the T.L.C. dye.
i. Flat scanner
ii. Digital camera
Now you are ready to start some testing!!
1.) Make sure samples are DRY and crumbly. The greater the moisture content the less accurate the T.L.C. method is.
2.) Weigh dry samples at a volume you can reproduce accurately. The volume should be less than 500mg for this test kit.
3.) Place samples in the 1.5mL tubes and add 1mL of the Extraction Solution. Crush and vigorously shake the samples. Let sit for ~5min.
T.L.C. Plate Prep:
1.) Only handle T.L.C. plates with gloved hands.
2.) Make marks on the plate with a sharp pencil at least ½” above the bottom of the plate. You do not want your samples in the Developing Solution when you place the T.L.C plates into the Developing Chamber.
3.) Load your desired volume of samples no less than 1 microliter no greater than 3 microliters. Be consistent with volumes loaded on the sample plate.
T.L.C. Plate Developing Prep:
1.) Place the contents of the developing solution bottle into the jar/developing chamber.
2.) Place the chamber on a level surface, and in an area where it will not be disturbed for ~15-20 minutes.
3.) Place T.L.C. plate into the chamber, carefully. Place the lid on the developing chamber, carefully. Let the plate run for ~15-20 minutes, or until the developing solution is ¼” from the top of the T.L.C. plate.
4.) Remove from the chamber and let air dry before using the T.L.C dye.
T.L.C. Dye Prep:
This is a DYE and WILL stain!!
1.) As the T.L.C. plates are running in the developing chamber, prepare your T.L.C. dye.
2.) Add 1mL water to the T.L.C. dye 1.5mL tube. Shake vigorously. Let set.
3.) Add 4mL of water to the T.L.C. sprayer.
4.) Transfer the 1mL T.L.C. dye concentrate to the T.L.C. sprayer. Shake vigorously. Let set.
5.) Use the Grow Buddy™ box as a spray chamber.
6.) One 5mL sprayer will dye 2 plates.
7.) Spray plates as you practiced.
8.) Let air dry.
Okay, you are almost done.
Capturing an Image:
By this point you have chosen a way to image your plates so you can send a picture to: firstname.lastname@example.org for free qualitative analysis. All results will be e-mailed back to you and the image of your slide will be annotated to show the novel cannabinoid compounds present in your samples.
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