Phyto–cannabinoids, also known as cannabinoids or exo–cannabinoids, are differentiated from endo-cannabinoids due to their production from enzymes in a plant opposed to being manufactured in a human, or better yet, a mammal. Cannabinoids come in many shapes and functions. Today, there are many known unique phytocannnabinoids and synthetic cannabinoids. Laboratories, like Montana Biotech, are currently assisting in cannabinoid testing. This service can help patients get the most from this ancient plant, cannabis.
Some of the >66 known Phytocannabinoids:
CBG (Cannabigerol): Non-psychoactive, sleep inducing. Anti-microbial. Lowers intra-ocular pressure (IOP) Glaucoma. Trends in Pharmacological Sciences Volume 30, Issue 10, October 2009, Pages 515-527
CBC (Cannabichromene): Sedative effect. Moderates effects of THC. Analgesic (pain relieving). Non-psychoactive. Trends in Pharmacological Sciences
Volume 30, Issue 10, October 2009, Pages 515-527
THCV (Tetrahydrocannabivarin): Stronger, faster “high” effect. Apatite suppressant. Euphoria, analgesic (pain relieving).
THC (tetrahydrocannabinol): Psychotropic. Analgesic (pain relieving). Apatite stimulant. Bronchial dilator. Lowers IOP/glaucoma.
CBD (Cannabidiol): Non-psychoactive. Reduces muscle spasms. Muscle relaxant. Analgesic (pain relieving) Trends in Pharmacological Sciences
Volume 30, Issue 10, October 2009, Pages 515-527
CBN (Cannabinol): Mildly psychoactive. Non-narcotic analgesic (pain relieving). Good indication of medications age. Trends in Pharmacological Sciences
Volume 30, Issue 10, October 2009, Pages 515-527
Determine percent THC CBD CBN T.L.C. Test Kit
Exploring the Endocannabinoid System Cannabinoids and their Therapeutic Potentials
Montana Biotech is a lab focusing on RE-discovering the science behind cannabis. Cannabinoids are the therapeutic compounds found in the cannabis genus. Both hemp and marijuana produce phyto-cannabinoids. The true difference between cannabis hemp and cannabis marijuana is the plants ability to produce, enzymaticaly, THCA. New discoveries have lead researchers to the hypothesis that it is the functionality of the enzyme, THCA synthase, not the absence of the gene coding for the enzyme that differentiates marijuana varieties from cannabis hemp varieties. Plant derived cannabinoids, phytocannabinoids, are created by the cannabis plant through a single enzyme catalyzed reaction. Each cannabinoid has a corresponding enzyme catalyzing their creation. CBN is the only known cannabinoid NOT derived from an enzymatic process; instead CBN is a degradation product, UV light exposure, heating, of THC.
Endocannabinoid System Network
Endocannabinoid System Explained, Dr. Melamede Ph.D professor at the University of Colorado at Colorado Springs
The endocannabinoid system refers to a group of neuromodulatory lipids and their receptors that are involved in a variety of physiological processes including appetite, pain-sensation, mood, and memory; it mediates the psychoactive effects of cannabis and, broadly speaking, includes:
- The cannabinoid receptors CB1 and CB2, two G protein-coupled receptors that are located in the central and peripheral nervous systems, respectively.
- The endogenous arachidonate-based lipids, anandamide (N-arachidonoylethanolamide, AEA) and 2-arachidonoylglycerol (2-AG); these are known as “endocannabinoids” and are physiological ligands for the cannabinoid receptors.
- The enzymes that synthesize and degrade the endocannabinoids. Unlike traditional neurotransmitters, endogenous cannabinoids are not stored in vesicles after synthesis, but are synthesized on demand (Rodriguez de Fonseca et al., 2004).[citation needed] However, some evidence suggests that a pool of synthesized endocannabinoids (namely, 2-AG) may exist without the requirement of on-demand synthesis.[1]
The endocannabinoid system has been studied using genetic and pharmacological methods. These studies have revealed a broad role for endocannabinoid signaling in a variety of physiological processes, including neuromodulator release,[2][3][4] motor learning,[5] synaptic plasticity,[6] appetite,[7] and pain sensation.[8]
Cannabis potency testing has come of age! Knowing the cannabinoid profile of a cannabis strain or variety will help providers of medical cannabis fit a patients’ condition to a medicine they will benefit from the best. Montana Biotech offers cannabinoid potency testing in Montana. There are two types of testing that can be employed by a laboratory or individual to analyze their cannabis, qualitative and quantitative.
Qualitative testing of cannabinoids:
When thinking of the difference between qualitative vs quantitative testing methods think of quality verses quantity. Thin layer chromatography, T.L.C., is a qualitative method of testing cannabis that provides a “picture” or “finger print” of the quality of phytocannabinoids present in a medical marijuana sample. It is an absent or present testing method; is CBD in the sample or not? T.L.C. is not a method to determine the amount of CBD in a cannabis sample as accurately as a quantitative testing method like gas chromatography, GC.
Quantitative testing of cannabinoids:
When thinking of the difference between quantitative vs qualitative testing methods think of quantity verses quality. Quantitative methods determine how much THC, CBD, CBN or other novel cannabinoids are present in a medical cannabis sample or cannabis infused preparation. Some of the well known quantitative methods are gas chromatography (GC), and high performance liquid chromatography (HPLC). Both methods are currently used by cannabis potency testing labs all over the U.S.
Qualitatively Test YOUR Cannabis at Home with Grow Buddy™
More information about cannabinoids and the diseases / illnesses /conditions they effect or treat:
Cannabinoids and diseases / illnesses /conditions they effect or treat
Cannabis Documentaries Cannabinoid Facts Marijuana Peer Reviewed Research Links
Cannabis TV Documentaries
Weed Wars on the Discovery Channel Episode 4 Season 1
Weed Wars on the Discovery Channel Episode 3 Season 1
Weed Wars on the Discovery Channel Episode 2 Season 1
Weed Wars on the Discovery Channel Episode 1 Season 1
Prohibition a Ken Burns Documentary on PBS
PBS FRONTLINE The Pot Republic
Montana PBS Special Clearing the Smoke: The Science of Cannabis
“She Shoulda Said ‘No’!” (also known as Wild Weed)
Marijuana ( Marihuana ) The Devil’s Weed Cannabis Propaganda film (1936)
Marihuana The Assassin of Youth 1935 Anti-Marijuana Propaganda Film
The BEST Cannabis / Marijuana Propaganda Films / Movies of ALL TIME!
Marijuana: A Chronic History Presented by the History Channel
TEA Party Eat your Heart Out!! The Bundy’s Were Here 1st!!
Links:
Find The Best Medical Marijuana in Montana Potency Tested
Exploring the Endocannabinoid System Cannabinoids and their Therapeutic Potentials
Hemp for Victory, 1942 U.S. WWII Propoganda Film, and State by State Industrial Hemp Laws
MMJ Clinics / Doctors Recommendations
MMJ Dispensaries/Caregivers: Find Them Here
MMJ Strains / Varieties: Find Them Here Potency Tested
Ed Rosenthal Runs For President in 2012? Stephen’s Colbert SuperPac Funding?
Fastest way to determine cannabis seedling sex!
Grow Buddy Thin Layer Chromatography THC, CBD test kit
Grow Buddy Instructional Video
Grow Buddy Replacement T.L.C. Supplies
Montana Biotech Products and Services
QUALTITATIVE vs. QAUNTITATIVE Analysis of Cannabis THC, CBD, CBN Cannabinoid Levels
Ed Rosenthal and the Party-Party’s Platform for America 2012!
A Little Ed History and Education! Wiki Style!
Peer Reviewed Research:
ADD /ADHD, Medical Marijuana and its potential Treatment.
Addiction Risk-Physical of using Medical Marijuana.
Alcoholism and Medical Marijuana.
ALS, Lou Gehrig’s Disease Medical Cannabis Therapy
Cannabis Marijuana Use and AMOTIVATIONAL SYNDROME
Alzheimer’s Disease Progression Prevention Cannabinoids
Medical Cannabis Marijuana and ANTI-INFLAMMATORY PROPERTIES of Cannabinoids
Medical Cannabis Marijuana and ANTI-INFLAMMATORY PROPERTIES of Cannabinoids
APPETITE STIMULANT Medical Marijuana Cannabis and It’s Effects on Hunger the Munchies
ARTHRITIS Cannabis Marijuana and the Effects of Cannabinoids for Treatment
ASTHMA Medical Cannabis / Marijuana and Cannabinoids Therapeutic Effects on Bronchodilation
Autism the Use of Medical Cannabis Marijuana Derived Cannabinoids for Treatment of Disorder
!!!Montana Biotech is NOW ACCEPTING PATIENTS (card holders)!!!
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Grow Buddy™, Test for THC, CBD, CBN at Home
Grow Buddy™ is a T.L.C. based method for analyzing cannabis for the presence of THC, CBD, CBN that ANYONE can perform at home! The Test is quick and fun! if you have brewed beer, wine, if you consider your self a “good” cook, you can perform a T.L.C. test for THC, CBD, CBN.
The control in this test was ~17% THC by volume… You can tell the size of the dots for Connie Chung (A) and (B) are larger. You can draw you own conclusions!
SEPARATION AND IDENTIFICATION OF CANNABIS COMPONENTS BY DIFFERENT PLANAR CHROMATOGRAPHY TECHNIQUES (TLC, AMD, OPLC)
N. GALAND*, D. ERNOUF**, F. MONTIGNY***, J. DOLLET* and J. POTHIER*
*UFR des Sciences Pharmaceutiques, Laboratoire de Pharmacognosie, 31 Avenue Monge F-3720 – TOURS
** UFR des Sciences Pharmaceutiques, Laboratoire de Toxicologie, 31 Avenue Monge, F 37200 – TOURS
***UFR des Sciences Pharmaceutiques, Plateau d’Analyses Chimiques et Biochimiques, 31 Avenue Monge,
F-37200 – TOURS
Abstract
The use of Cannabis is illicit in numerous countries and the increasing consumption has led to a multiplication of scientific studies. New methods of planar chromatography such as Automated Multiple Development (AMD) and Optimum Performance Laminar Chromatography (OPLC) techniques can be used as a substitute for the traditional TLC for the identification and quantification of the Indian hemp components. Each method offers its own advantage: high resolution with neither diffusion nor spot stretching for AMD, speed, efficiency and the possibility of working in semi-preparative mode for OPLC.
Cannabis_components_by_TLC_OPLC_AMD
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