Instructional Video: Grow Buddy™ at Home THC, CBD, CBN, Thin Layer Chromatography, TLC , Test Kit

Start Here!

1.) Read manual thoroughly before analyzing your samples!

2.) Grow Buddy™ contains solvents, such as, chloroform. Bottles are labeled with safety precautions and hazard labels. Handle with care and wear gloves always.

3.) Be sure to rinse and clean the sprayer thoroughly before storage. Keep a small volume of water in the sprayer (~1mL). Keep the pump primed. This will extend the life of your sprayer.

4.) Practice with sprayer before using on T.L.C. plates. Just use water and a piece of paper to practice first.

5.) ALWAYS WEAR GLOVES WHILE USING THE KIT!

6.) Colors will fade on the T.L.C. plates. Image the plates at least 10 minutes after spraying with the T.L.C. dye. A longer wait can result in unreliable data.

7.) Keep 1mL pipettes separate so as to not cross contaminate your solutions.

8.) While using the capillary tubes, have ethanol or rubbing alcohol on hand to place the tubes in for cleaning and re-use!

9.) Heating the plates allows analysis of decarboylated acids (bio-active) cannabinoids. Non-heated plates will represent cannabinoid acids present in the sample tested. Some cannabinoids will be present in the acid orientation at greater concentrations than the decarboylated form.

So, off you go! And, good luck! Montana Biotech® is there to answer your questions, anytime!

Cannabinoid Acids Comparison Assay

Area Prep:

1.) Remove all items from the area not needed for your testing.

2.) Collect materials not included in the kit needed for testing.

a) Thermometer

b) A scale accurate to .1g

c) Timer, or some way to keep track of elapsed time

d) Some way to capture an image of the T.L.C. plates after being sprayed with the T.L.C. dye.

i. Flat scanner

ii. Digital camera

Now you are ready to start some testing!!

WEAR GLOVES!!

Sample Prep:

1.) Make sure samples are DRY and crumbly. The greater the moisture content the less accurate the T.L.C. method is.

2.) Weigh dry samples at a volume you can reproduce accurately. The volume should be less than 500mg for this test kit.

3.) Place samples in the 1.5mL tubes and add 1mL of the Extraction Solution. Crush and vigorously shake the samples. Let sit for ~5min.

T.L.C. Plate Prep:

1.) Only handle T.L.C. plates with gloved hands.

2.) Make marks on the plate with a sharp pencil at least ½” above the bottom of the plate. You do not want your samples in the Developing Solution when you place the T.L.C plates into the Developing Chamber.

3.) Load your desired volume of samples no less than 1 microliter no greater than 3 microliters. Be consistent with volumes loaded on the sample plate.

T.L.C. Plate Developing Prep:

1.) Place the contents of the developing solution bottle into the jar/developing chamber.

2.) Place the chamber on a level surface, and in an area where it will not be disturbed for ~15-20 minutes.

3.) Place T.L.C. plate into the chamber, carefully. Place the lid on the developing chamber, carefully. Let the plate run for ~15-20 minutes, or until the developing solution is ¼” from the top of the T.L.C. plate.

4.) Remove from the chamber and let air dry before using the T.L.C dye.

T.L.C. Dye Prep:

This is a DYE and WILL stain!!

1.) As the T.L.C. plates are running in the developing chamber, prepare your T.L.C. dye.

2.) Add 1mL water to the T.L.C. dye 1.5mL tube. Shake vigorously. Let set.

3.) Add 4mL of water to the T.L.C. sprayer.

4.) Transfer the 1mL T.L.C. dye concentrate to the T.L.C. sprayer. Shake vigorously. Let set.

5.) Use the Grow Buddy™ box as a spray chamber.

6.) One 5mL sprayer will dye 2 plates.

7.) Spray plates as you practiced.

8.) Let air dry.

Okay, you are almost done.

Capturing an Image:

By this point you have chosen a way to image your plates so you can save and analyze the data at your leisure. Have the plate imaged at least 15 min after dying. If not, results are unreliable.

Bio-Active Cannabinoid Comparison Assay

Area Prep:

1.) Remove all items from the area not needed for your testing.

2.) Collect materials not included in the kit needed for testing.

a) Thermometer

b) A scale accurate to .1g

c) Timer, or some way to keep track of elapsed time

d) Some way to capture an image of the T.L.C. plates after being sprayed with the T.L.C. dye.

i. Flat scanner

ii. Digital camera

Now you are ready to start some testing!!

WEAR GLOVES!!

Sample Prep:

1.) Make sure samples are DRY and crumbly. The greater the moisture content the less accurate the T.L.C. method is.

2.) Weigh dry samples at a volume you can reproduce accurately. The volume should be less than 500mg for this test kit.

3.) Place samples in the 1.5mL tubes and add 1mL of the Extraction Solution. Crush and vigorously shake the samples. Let sit for ~5min.

T.L.C. Plate Prep:

1.) Only handle T.L.C. plates with gloved hands.

3.) Load your desired volume of samples no less than 1 microliter no greater than 3 microliters. Be consistent with volumes loaded on the sample plate.

T.L.C. Plate Developing Prep:

1.) Place the contents of the developing solution bottle into the jar/developing chamber.

2.) Place the chamber on a level surface, and in an area where it will not be disturbed for ~15-20 minutes.

2A.) Heat plates in a 300°F preheated oven for ~3.5 minutes. Remove from heat and let cool.

4.) Remove from the chamber and let air dry before using the T.L.C dye.

T.L.C. Dye Prep:

This is a DYE and WILL stain!!

1.) As the T.L.C. plates are running in the developing chamber, prepare your T.L.C. dye.

2.) Add 1mL water to the T.L.C. dye 1.5mL tube. Shake vigorously. Let set.

3.) Add 4mL of water to the T.L.C. sprayer.

4.) Transfer the 1mL T.L.C. dye concentrate to the T.L.C. sprayer. Shake vigorously. Let set.

5.) Use the Grow Buddy™ box as a spray chamber.

6.) One 5mL sprayer will dye 2 plates.

7.) Spray plates as you practiced.

8.) Let air dry.

Okay, you are almost done.

Capturing an Image:

By this point you have chosen a way to image your plates so you can save and analyze the data at your leisure. Have the plate imaged at least 15 min after dying. If not, results are unreliable.

The Grow Buddy™ at home THC testing kit is just $100.00 plus $3.50 for shipping and handling in the U.S. Order yours today!

The Grow Buddy™ at home THC testing kit is just $100.00 plus $50.00 for International Orders, shipping and handling. Order yours today! Grow Buddy T.L.C. Cannabinoids THC, CBD Test Kit Instructions Dutch Translation

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