Montana BioTech is proud to introduce the Plant DNA Collection Kit™. This kit has many functions! The small time patient, to the large scale farmer can gain from this kit / service! Here is how it works!
Montana Biotech currently offers hermaphroditetesting and sexing of Cannabis 1-2 weeks after germination! So, if you are looking for the FASTEST method to sex your medical marijuana / cannabis seedlings, call on Montana Biotech!Male / Female and Hermaphrodite Screening, or Genetic Profiling:
In today’s environment of high competition in the medical marijuana industry, a way to distinguish what you have from your competitors is essential! This kit can be used to create a uniquegeneticprofile of your hybrid! This PCR, gel electrophoresis based test can provide you a “picture” of YOUR plantsgeneticmake-up! With this info in hand, it is easier to obtain a trademark on your variety / strain with the U.S. Patent and Trademark Office.
Another advantage that can be gained with the Plant DNA Collection Kit™ is TIME! TIME from seed, to clone, to harvest! At some point we all need to start from seed, whether it is starting a new variety / strain, or creating your own hybrid. It would be great to be able to weed the males from the females to increaseproductivity! This can be obtained with male / female sexing! Male / female sexing is a PCR based test. 1-2 weeks after germination of the seed, KNOW if it’s male or female by its GENES. NO GUESS WORK. By time that seedling is ready for cloning, KNOW that it is a FEMALE, and move on!! Maintain the phenotype without having to re-vegetate the plant, our having unwanted clones reducing cost!
Have you ever wanted to test your cannabis plant to see if it is a hermaphrodite? Well, Montana Biotech has a test for that! A cannabis plant will express hermaphroditic tendencies from environmental stresses or because of a genetic predisposition to express monoecious traits. A monoecious cannabisplant is a TRUE hermaphrodite. This cannabis plant will express male and female flower structures on the same plant. This is the hermaphrodite to be aware of! A true hermaphrodite sexes as a female early in the flowering cycle. A true hermaphrodite is a ticking time bomb! She/it will seed everything in your flowering room! Environmentally induced hermaphroditecannabis plants can not be tested for, at this time, and do not pose the same risk to the rest of the room. These hermaphrodites are not “true” hermaphrodites and usually pollinate themselves. ANY cannabisplant can be induced to revert to a hermaphroditic state by stress. By stressing a cannabis plant, you have tapped into its inherent survival programming. Montana Biotech now offers a Polymerase Chain Reaction (PCR) based test for hermaphroditescreening of cannabis plants. KNOW if your cannabis plant has the TRUE hermaphrodite trait before you flower it! Our motto is, “Know before they show…”
!!!Montana Biotech is NOW ACCEPTING PATIENTS!!!
If you have a qualifying medical condition, but cant afford the cost of MMJ registration, we may be able to help.
Wholesale prices are available for all of our products including a 90 day buy back guarantee. Please feel free to contact us 24/7 with any questions.
UPGRADE for the Grow Buddy™ home THC, CBD, CBN, and other novel cannabinoids test kit. JustTLC is a software program enabling an individual to take thin layer chromatography plate results and get a more quantitative picture of your samples. JustTLC can be used in conjunction with the Grow Buddy™ to help discover what makes the medicine in your closet, medicine.
Grow Buddy THC CBD Home Test Kit Instructions English
Area Prep:
1.) Remove all items from the area not needed for your testing.
2.) Collect materials not included in the kit needed for testing.
a) Thermometer
b) A scale accurate to .1g
c) Timer, or some way to keep track of elapsed time
d) Some way to capture an image of the T.L.C. plates after being sprayed with the T.L.C. dye.
i. Flat scanner
ii. Digital camera
Now you are ready to start some testing!!
WEAR GLOVES!!
Sample Prep:
1.) Make sure samples are DRY and crumbly. The greater the moisture content the less accurate the T.L.C. method is.
2.) Weigh dry samples at a volume you can reproduce accurately. The volume should be less than 500mg for this test kit.
3.) Place samples in the 1.5mL tubes and add 1mL of the ExtractionSolution. Crush and vigorously shake the samples. Let sit for ~5min.
T.L.C. Plate Prep:
1.) Only handle T.L.C. plates with gloved hands.
2.) Make marks on the plate with a sharp pencil at least ½” above the bottom of the plate. You do not want your samples in the DevelopingSolution when you place the T.L.C plates into the DevelopingChamber.
3.) Load your desired volume of samples no less than 1 microliter no greater than 3 microliters. Be consistent with volumes loaded on the sample plate.
T.L.C. Plate Developing Prep:
1.) Place the contents of the developing solution bottle into the jar/developing chamber.
2.) Place the chamber on a level surface, and in an area where it will not be disturbed for ~15-20 minutes.
3.) Place T.L.C. plate into the chamber, carefully. Place the lid on the developing chamber, carefully. Let the plate run for ~15-20 minutes, or until the developing solution is ¼” from the top of the T.L.C. plate.
4.) Remove from the chamber and let air dry before using the T.L.C dye.
T.L.C. Dye Prep:
This is a DYE and WILL stain!!
1.) As the T.L.C. plates are running in the developing chamber, prepare your T.L.C. dye.
2.) Add 1mL water to the T.L.C. dye 1.5mL tube. Shake vigorously. Let set.
3.) Add 4mL of water to the T.L.C. sprayer.
4.) Transfer the 1mL T.L.C. dye concentrate to the T.L.C. sprayer. Shake vigorously. Let set.
5.) Use the Grow Buddy™ box as a spray chamber.
6.) One 5mL sprayer will dye 2 plates.
7.) Spray plates as you practiced.
8.) Let air dry.
Okay, you are almost done.
Capturing an Image:
By this point you have chosen a way to image your plates so you can send a picture to: montanabiotech@gmail.com for free qualitative analysis. All results will be e-mailed back to you and the image of your slide will be annotated to show the novel cannabinoid compounds present in your samples.
!!!Montana Biotech is NOW ACCEPTING PATIENTS!!!
If you have a qualifying medical condition, but cant afford the cost of MMJ registration, we may be able to help.
Wholesale prices are available for all of our products including a 90 day buy back guarantee. Please feel free to contact us 24/7 with any questions.
Grow Buddy™ is a T.L.C. based method for analyzing cannabis for the presence of THC, CBD, CBN that ANYONE can perform at home! The Test is quick and fun! if you have brewed beer, wine, if you consider your self a “good” cook, you can perform a T.L.C. test for THC, CBD, CBN.
TLC, UNODC (United Nations Office on Drug and Crime)
TLC was the method of choice for this study as it provides a means of rapidly and visuallyanalysing a number of samplessimultaneously. Unlike the comparison of two samples in order to assess whether they are the same (or different), when quantitativeanalysis is essential, assessment of origin is qualitative, since the sample under study and the referencesamples are, in fact, different. There is thus little point in using more powerfultechniques such as high-performance liquid chromatography as the quantitativeinformation provided is likely to confuse the picture. The TLCsystem was designed to give information on the cannabivarins in addition to the more commonly studied cannabinoids. The principal cannabinoids observed using this system are given in table 2. Sample preparation and detection were as previously described [ 47] [ 48] . The solvent system used was a mixture of chloroform (ethanol-free) and l,l-dichloroethane (15:10) with simple ascending TLC on 10 x 20 cm silica gel pre-coated plates with a layer thickness of 0.25 mm (E. Merck, Darmstadt, G.F.R., Art. No. 5729) [ 46] . Each extract was analysed within one hour of preparation. Twentydifferent samples of herbalcannabis and cannabisresin from several differentcountries were subjected to hot-solvent extraction to simulate the illicitpreparation of liquidcannabis. The resulting chromatograms were compared with those of the startingmaterial..
The control in this test was ~17% THC by volume… You can tell the size of the dots for Connie Chung (A) and (B) are larger. You can draw you own conclusions!
SEPARATION AND IDENTIFICATION OF CANNABIS COMPONENTS BY DIFFERENT PLANAR CHROMATOGRAPHY TECHNIQUES (TLC, AMD, OPLC)
N. GALAND*, D. ERNOUF**, F. MONTIGNY***, J. DOLLET* and J. POTHIER*
*UFR des Sciences Pharmaceutiques, Laboratoire de Pharmacognosie, 31 Avenue Monge F-3720 – TOURS
** UFR des Sciences Pharmaceutiques, Laboratoire de Toxicologie, 31 Avenue Monge, F 37200 – TOURS
***UFR des Sciences Pharmaceutiques, Plateau d’Analyses Chimiques et Biochimiques, 31 Avenue Monge,
F-37200 – TOURS
Abstract
The use of Cannabis is illicit in numerous countries and the increasing consumption has led to a multiplication of scientific studies. New methods of planar chromatography such as Automated Multiple Development (AMD) and Optimum Performance Laminar Chromatography (OPLC) techniques can be used as a substitute for the traditional TLC for the identification and quantification of the Indian hemp components. Each method offers its own advantage: high resolution with neither diffusion nor spot stretching for AMD, speed, efficiency and the possibility of working in semi-preparative mode for OPLC.
Have you ever found it hard to findcannabis documentaries cannabinoids facts or peer reviewed research to share with friends, family members, or associates? Well, look no further! Montana Biotech has been compiling the resources for you, the patient, provider, individual, or researcher. Feel free to share these links as you feel fit. You took the time to find these cannabis documentaries, cannabinoid facts, and marijuana peer reviewed research links here, no need to look further. As new content is found and shared, Montana Biotech will find the time to share.
1.) Verwijderen alle items uit het gebied niet nodig voor het testen.
2.) Verzamelen materialen niet inbegrepen in de kit nodig voor het testen.
a) thermometer
b) A scale accurate to .1g
c) timer, of andere manier voor het bijhouden van verstreken tijd
d) andere manier een opname van de T.L.C. platen na wordt gespoten met de T.L.C. kleurstof.
i. Platte scanner
ii. Digitale camera
Nu bent u klaar om te beginnen wat testen!
DRAAG HANDSCHOENEN!
Monster Prep:
1.) Zorg ervoor dat monsters zijn droog en kruimelig. Hoe groter het vochtgehalte de minder nauwkeurig de T.L.C. methode is.
2.) Weeg droge monsters op een volume dat u nauwkeurig kunt reproduceren. Niet meer dan 500 mg voor deze kit.
3.) Leg monsters in de buizen 1,5 mL en voeg 1 mL van de extractieoplossing.
Crush en schud krachtig de monsters. Laat het zitten voor ~ 5 min.
T.L.C. plaat Prep:
1.) Enige greep T.L.C. platen met gehandschoende handen.
2.) Maken merken op de plaat met een scherp potlood ten minste ½ “boven de bodem van de plaat. U wilt niet uw monsters in de oplossing ontwikkelen wanneer u de T.L.C platen in de bedwelmingsruimte ontwikkelen plaatst.
3.) Laden uw gewenste volume van monsters niet minder dan 1 microliter niet groter is dan 3 microliters. Verenigbaar moet zijn met volumes op de steekproef plaat geladen.
T.L.C. plaat Prep ontwikkelen:
1.) Plaats de inhoud van de ontwikkelingslanden oplossing fles in de pot/ontwikkelen kamer.
2.) Plaats de zaal op een vlakke ondergrond, en in een gebied waar het zal niet worden verstoord voor ~ 15-20 minuten.
3.) Plaats T.L.C. plaat in de vergaderzaal, zorgvuldig. Plaats het deksel op de derde kamer, zorgvuldig. Laat de plaat uitvoeren voor ~ 15-20 minuten, of totdat de derde oplossing ¼ “vanaf de bovenkant van de T.L.C. plaat is.
4.) Verwijderen uit de kamer en laat lucht droog is voordat u de T.L.C kleurstof.
T.L.C. kleurstof Prep:
Dit is een KLEURSTOF en zal vlekken!
1.) Als de T.L.C. platen worden uitgevoerd in de derde kamer, uw T.L.C. kleurstof voor te bereiden.
2.) Toevoegen 1 mL water aan de T.L.C. kleurstof 1,5 mL-buis. Schud krachtig. Laat instellen.
4.) Overdracht de 1 mL T.L.C. kleurstof concentreren aan de sproeier T.L.C.. Schud krachtig. Laat instellen.
5.) Het gebruik het vak groeien Buddy ™ als een spray-kamer.
6.) Één 5 mL sproeier 2 platen zal kleurstof.
7.) Spray platen als u beoefend.
8.) Laat lucht droog.
Oke, bent je bijna klaar.
Maken van een opname:
Door dit punt hebt u een manier om het imago van uw platen, zodat u kunt opslaan en analyseren van de gegevens op uw gemak. De plaat hebben beeld ten minste 15 min. na het sterven. Als dat niet het geval is, resultaten onbetrouwbaar zijn.
Bio-actieve cannabinoïde vergelijking Assay
Gebied Prep:
1.) Verwijderen alle items uit het gebied niet nodig voor het testen.
2.) Verzamelen materialen niet inbegrepen in de kit nodig voor het testen.
a) thermometer
b) A scale accurate to .1g
c) timer, of andere manier voor het bijhouden van verstreken tijd
d) andere manier een opname van de T.L.C. platen na wordt gespoten met de T.L.C. kleurstof.
i. Platte scanner
ii. Digitale camera
Nu bent u klaar om te beginnen wat testen!
DRAAG HANDSCHOENEN!
Monster Prep:
1.) Zorg ervoor dat monsters zijn droog en kruimelig. Hoe groter het vochtgehalte de minder nauwkeurig de T.L.C. methode is.
2.) Weeg droge monsters op een volume dat u nauwkeurig kunt reproduceren. Niet meer dan 500 mg voor deze kit.
3.) Leg monsters in de buizen 1,5 mL en voeg 1 mL van de extractieoplossing.
Crush en schud krachtig de monsters. Laat het zitten voor ~ 5 min.
T.L.C. plaat Prep:
1.) Enige greep T.L.C. platen met gehandschoende handen.
2.) Maken merken op de plaat met een scherp potlood ten minste ½ “boven de bodem van de plaat. U wilt niet uw monsters in de oplossing ontwikkelen wanneer u de T.L.C platen in de bedwelmingsruimte ontwikkelen plaatst.
3.) Laden uw gewenste volume van monsters niet minder dan 1 microliter niet groter is dan 3 microliters. Verenigbaar moet zijn met volumes op de steekproef plaat geladen.
T.L.C. plaat Prep ontwikkelen:
1.) Plaats de inhoud van de ontwikkelingslanden oplossing fles in de pot/ontwikkelen kamer.
2.) Plaats de zaal op een vlakke ondergrond, en in een gebied waar het zal niet worden verstoord voor ~ 15-20 minuten.
2A.) warmte platen in een voorverwarmde oven van 300 ° F voor ~3.5 minuten. Verwijder uit hitte en laat koel.
3.) Plaats T.L.C. plaat in de vergaderzaal, zorgvuldig. Plaats het deksel op de derde kamer, zorgvuldig. Laat de plaat uitvoeren voor ~ 15-20 minuten, of totdat de derde oplossing ¼ “vanaf de bovenkant van de T.L.C. plaat is.
4.) Verwijderen uit de kamer en laat lucht droog is voordat u de T.L.C kleurstof.
T.L.C. kleurstof Prep:
Dit is een KLEURSTOF en zal vlekken!
1.) Als de T.L.C. platen worden uitgevoerd in de derde kamer, uw T.L.C. kleurstof voor te bereiden.
2.) Toevoegen 1 mL water aan de T.L.C. kleurstof 1,5 mL-buis. Schud krachtig. Laat instellen.
4.) Overdracht de 1 mL T.L.C. kleurstof concentreren aan de sproeier T.L.C.. Schud krachtig. Laat instellen.
5.) Het gebruik het vak groeien Buddy ™ als een spray-kamer.
6.) Één 5 mL sproeier 2 platen zal kleurstof.
7.) Spray platen als u beoefend.
8.) Laat lucht droog.
Oke, bent je bijna klaar.
Maken van een opname:
Door dit punt hebt u een manier om het imago van uw platen, zodat u kunt opslaan en analyseren van de gegevens op uw gemak. De plaat hebben beeld ten minste 15 min. na het sterven. Als dat niet het geval is, resultaten onbetrouwbaar zijn.
The Grow Buddy™at home THC testing kit is just $100.00 plus $50.00 for International Orders, shipping and handling. Order yours today!
!!!Montana Biotech is NOW ACCEPTING PATIENTS (card holders)!!!
Contact us to be YOURProvider/Marijuana Infused Products Provider (MIPP)
Call: 406-600-6871
This is an industry first! Other kits exist in the market, but only Montana Biotech offers our clients superior service with laboratory grade results for both chemical and genetic analysis.
How it works:
Past clients will be receiving an email which will include an order’s Transaction ID Number. This will allow us to make this offer retroactive for all past clients. New clients will receive an order’s Transaction ID Number sticker for every slide sold in our Grow buddy kits, as well as from our resupply list.
Once you have developed your slide and the T.L.C. dye has dried revealing your unique cannabinoid profile, simply place the slide on a dark background, preferably black, making sure your order’s Transaction ID Number is clearly in frame and legible. You will take a digital photo of your slide and send it to Montanabiotech@gmail.com with the subject line of T.L.C Plate. Please only send photos in Jpeg format.
Within 24 hours you will receive your qualitative results via reply e-mail, or if we are unable to review your photo for any reason, you will at least be contacted via reply e-mail with further questions or instructions. As always for those seeking quantitative results we recommend you try a free 30 day trial ofJustTLC Thin Layer Chromatography (T.L.C.) analytical software.
Call Montana Biotech today for more information on setting up a Grow Room, Help or consultation!
T.L.C., thin layer chromatography, have you ever tried it? It is not as bad as it seems! And, T.L.C. has just gotten EASIER! T.L.C. methods require many different pieces, parts, accessories and supplies to be able to conduct the testing. Montana Biotech is proud to introduce replacementthin layer chromatography, T.L.C., pieces, parts,accessories and supplies for Grow Buddy thin layer chromatography at home THC, CBD, CBN, cannabinoid finger print testing kit!! NOW, the testing does not have to STOP because you need more TLC plates, TLC dye, or a NEW TLC sprayer! TLC Capillarytubes are easy to break or loose! More 1.5mL extraction tubes equals MORE samples you can test! And, of course, MORE ExtractionSolvent, and Developing Solution assures further testing bliss. (1.) Replacement Thin Layer Chromatography, TLC, Plates:
Have you ever found it hard to findcannabis documentaries cannabinoids facts or peer reviewed research to share with friends, family members, or associates? Well, look no further! Montana Biotech has been compiling the resources for you, the patient, provider, individual, or researcher. Feel free to share these links as you feel fit. You took the time to find these cannabis documentaries, cannabinoid facts, and marijuana peer reviewed research links here, no need to look further. As new content is found and shared, Montana Biotech will find the time to share.
Phyto–cannabinoids, also known as cannabinoids or exo–cannabinoids, are differentiated from endo-cannabinoids due to their production from enzymes in a plant opposed to being manufactured in a human, or better yet, a mammal. Cannabinoids come in many shapes and functions. Today, there are many known unique phytocannnabinoids and synthetic cannabinoids. Laboratories, like Montana Biotech, are currently assisting in cannabinoidtesting. This service can help patients get the most from this ancient plant, cannabis.
Montana Biotech is a lab focusing on RE-discovering the science behind cannabis. Cannabinoids are the therapeutic compounds found in the cannabis genus. Both hemp and marijuana produce phyto-cannabinoids. The true difference between cannabishemp and cannabis marijuana is the plants ability to produce, enzymaticaly, THCA. New discoveries have lead researchers to the hypothesis that it is the functionality of the enzyme, THCAsynthase, not the absence of the gene coding for the enzyme that differentiates marijuana varieties from cannabishemp varieties. Plant derived cannabinoids, phytocannabinoids, are created by the cannabis plant through a single enzyme catalyzed reaction. Each cannabinoid has a corresponding enzyme catalyzing their creation. CBN is the only known cannabinoid NOT derived from an enzymatic process; instead CBN is a degradation product, UV light exposure, heating, of THC.
Endocannabinoid System Network
Endocannabinoid System Explained, Dr. Melamede Ph.D professor at the University of Colorado at Colorado Springs
The endocannabinoid system refers to a group of neuromodulatory lipids and their receptors that are involved in a variety of physiological processes including appetite, pain-sensation, mood, and memory; it mediates the psychoactive effects of cannabis and, broadly speaking, includes:
The enzymes that synthesize and degrade the endocannabinoids. Unlike traditional neurotransmitters, endogenous cannabinoids are not stored in vesicles after synthesis, but are synthesized on demand (Rodriguez de Fonseca et al., 2004).[citation needed] However, some evidence suggests that a pool of synthesized endocannabinoids (namely, 2-AG) may exist without the requirement of on-demand synthesis.[1]
The endocannabinoid system has been studied using genetic and pharmacological methods. These studies have revealed a broad role for endocannabinoid signaling in a variety of physiological processes, including neuromodulator release,[2][3][4]motor learning,[5]synaptic plasticity,[6]appetite,[7] and pain sensation.[8]
Cannabis potency testing has come of age! Knowing the cannabinoid profile of a cannabis strain or variety will help providers of medical cannabis fit a patients’ condition to a medicine they will benefit from the best. Montana Biotech offers cannabinoid potency testing in Montana. There are two types of testing that can be employed by a laboratory or individual to analyze their cannabis, qualitative and quantitative.
When thinking of the difference between qualitative vs quantitativetesting methods think of quality verses quantity. Thin layer chromatography, T.L.C., is a qualitative method of testingcannabis that provides a “picture” or “finger print” of the quality of phytocannabinoidspresent in a medical marijuana sample. It is an absent or present testing method; is CBD in the sample or not? T.L.C. is not a method to determine the amount of CBD in a cannabis sample as accurately as a quantitativetesting method like gas chromatography, GC.
When thinking of the difference between quantitative vs qualitativetesting methods think of quantity verses quality. Quantitative methods determine how much THC, CBD, CBN or other novel cannabinoids are present in a medical cannabis sample or cannabis infused preparation. Some of the well known quantitative methods are gas chromatography (GC), and high performance liquid chromatography (HPLC). Both methods are currently used by cannabispotency testing labs all over the U.S.
Grow Buddy™ is a T.L.C. based method for analyzing cannabis for the presence of THC, CBD, CBN that ANYONE can perform at home! The Test is quick and fun! if you have brewed beer, wine, if you consider your self a “good” cook, you can perform a T.L.C. test for THC, CBD, CBN.
The control in this test was ~17% THC by volume… You can tell the size of the dots for Connie Chung (A) and (B) are larger. You can draw you own conclusions!
SEPARATION AND IDENTIFICATION OF CANNABIS COMPONENTS BY DIFFERENT PLANAR CHROMATOGRAPHY TECHNIQUES (TLC, AMD, OPLC)
N. GALAND*, D. ERNOUF**, F. MONTIGNY***, J. DOLLET* and J. POTHIER*
*UFR des Sciences Pharmaceutiques, Laboratoire de Pharmacognosie, 31 Avenue Monge F-3720 – TOURS
** UFR des Sciences Pharmaceutiques, Laboratoire de Toxicologie, 31 Avenue Monge, F 37200 – TOURS
***UFR des Sciences Pharmaceutiques, Plateau d’Analyses Chimiques et Biochimiques, 31 Avenue Monge,
F-37200 – TOURS
Abstract
The use of Cannabis is illicit in numerous countries and the increasing consumption has led to a multiplication of scientific studies. New methods of planar chromatography such as Automated Multiple Development (AMD) and Optimum Performance Laminar Chromatography (OPLC) techniques can be used as a substitute for the traditional TLC for the identification and quantification of the Indian hemp components. Each method offers its own advantage: high resolution with neither diffusion nor spot stretching for AMD, speed, efficiency and the possibility of working in semi-preparative mode for OPLC.
Montana BioTech specializes in cannabis potency lab testing. Botanical Genetic profiling is now offered to help with the identifying and categorizing of new varieties / cultivars / strains for the medical cannabis industry. Montana BioTech can genetically screen cannabis for male or females 1-2 weeks after germination! Montana BioTech can also certify seed as feminized. Cannabisvarieties / cultivars / strains will be added to our data base and made public as soon as samples are scrutinized and released by the grower. Montana BioTech is willing to help patients and caregivers achieve the best results from their medicine. What else can I say about botanical genetic analysis?
Montana BioTech is proud to introduce the Plant DNA Collection Kit™. This kit has many functions! The small time patient, to the large scale farmer can gain from this kit / service!
In today’s environment of high competition in the medical marijuana industry, a way to distinguish what you have from your competitors is essential! This kit can be used to create a uniquegeneticprofile of your hybrid! This PCR, gel electrophoresis based test can provide you a “picture” of YOUR plantsgeneticmake-up! With this info in hand, it is easier to obtain a trademark on your variety / strain with the U.S. Patent and Trademark Office.
Another advantage that can be gained with the Plant DNA Collection Kit™ is TIME! TIME from seed, to clone, to harvest! At some point we all need to start from seed, whether it is starting a new variety / strain, or creating your own hybrid. It would be great to be able to weed the males from the females to increaseproductivity! This can be obtained with male / female sexing! Male / female sexing is a PCR based test. 1-2 weeks after germination of the seed, KNOW if it’s male or female by its GENES. NO GUESS WORK. By time that seedling is ready for cloning, KNOW that it is a FEMALE, and move on!! Maintain the phenotype without having to re-vegetate the plant, our having unwanted clones reducing cost!
Montana BioTech is committed to providing services and products NO OTHER LABORATORY CAN or IS!!
UPGRADE for the Grow Buddy™ home THC, CBD, CBN, and other novel cannabinoidstest kit. JustTLC is a software program enabling an individual to take thin layer chromatography plate results and get a more quantitative picture of your samples. JustTLC can be used in conjunction with the Grow Buddy™ to help discover what makes the medicine in your closet, medicine.
When used as a suite, Grow Buddy, JustTLC 30 day FREE trial, and the Montana BiotechTHC, CBN Standard, a TRUE analysis of your cannabis can be done in the convenience of your home for around $6 per sample! If you need Customer services, they are just a phone call away.
Montana Biotech is a laboratory focusing on re-discovering the science behind cannabis. We offer: Potency Analysis testing, we also offer a way to test for THC, CBD and other novel cannabinoids at home with the Grow BuddyT.L.C. test kit. Grow Buddy is a way to qualitatively, vs quantitatively, analyze your medicine. We do not currently offer out of state testing, even by mail. Montana Biotech can determine if your cannabis seedling is male or female 1-2 weeks after germination, or if your plant is a TrueHermaphrodite using the Plant DNA Collection Kit and a PCR, gel electrophoresis method. The DNA Collection Kit can also be used for genetic profiling, i.e. percent sativa, or Blueberry vs Skunk #1.
!!!Montana Biotech is NOW ACCEPTING PATIENTS!!!
If you have a qualifying medical condition, but cant afford the cost of MMJ registration, we may be able to help.
Wholesale prices are available for all of our products including a 90 day buy back guarantee. Please feel free to contact us 24/7 with any questions.
Grow Buddy™ is a T.L.C. based method for analyzing cannabis for the presence of THC, CBD, CBN that ANYONE can perform at home! The Test is quick and fun! if you have brewed beer, wine, if you consider your self a “good” cook, you can perform a T.L.C. test for THC, CBD, CBN.
TLC, UNODC (United Nations Office on Drug and Crime)
TLC was the method of choice for this study as it provides a means of rapidly and visuallyanalysing a number of samplessimultaneously. Unlike the comparison of two samples in order to assess whether they are the same (or different), when quantitativeanalysis is essential, assessment of origin is qualitative, since the sample under study and the referencesamples are, in fact, different. There is thus little point in using more powerfultechniques such as high-performance liquid chromatography as the quantitativeinformation provided is likely to confuse the picture. The TLCsystem was designed to give information on the cannabivarins in addition to the more commonly studied cannabinoids. The principal cannabinoids observed using this system are given in table 2. Sample preparation and detection were as previously described [ 47] [ 48] . The solvent system used was a mixture of chloroform (ethanol-free) and l,l-dichloroethane (15:10) with simple ascending TLC on 10 x 20 cm silica gel pre-coated plates with a layer thickness of 0.25 mm (E. Merck, Darmstadt, G.F.R., Art. No. 5729) [ 46] . Each extract was analysed within one hour of preparation. Twentydifferent samples of herbalcannabis and cannabisresin from several differentcountries were subjected to hot-solvent extraction to simulate the illicitpreparation of liquidcannabis. The resulting chromatograms were compared with those of the startingmaterial..
The control in this test was ~17% THC by volume… You can tell the size of the dots for Connie Chung (A) and (B) are larger. You can draw you own conclusions!
SEPARATION AND IDENTIFICATION OF CANNABIS COMPONENTS BY DIFFERENT PLANAR CHROMATOGRAPHY TECHNIQUES (TLC, AMD, OPLC)
N. GALAND*, D. ERNOUF**, F. MONTIGNY***, J. DOLLET* and J. POTHIER*
*UFR des Sciences Pharmaceutiques, Laboratoire de Pharmacognosie, 31 Avenue Monge F-3720 – TOURS
** UFR des Sciences Pharmaceutiques, Laboratoire de Toxicologie, 31 Avenue Monge, F 37200 – TOURS
***UFR des Sciences Pharmaceutiques, Plateau d’Analyses Chimiques et Biochimiques, 31 Avenue Monge,
F-37200 – TOURS
Abstract
The use of Cannabis is illicit in numerous countries and the increasing consumption has led to a multiplication of scientific studies. New methods of planar chromatography such as Automated Multiple Development (AMD) and Optimum Performance Laminar Chromatography (OPLC) techniques can be used as a substitute for the traditional TLC for the identification and quantification of the Indian hemp components. Each method offers its own advantage: high resolution with neither diffusion nor spot stretching for AMD, speed, efficiency and the possibility of working in semi-preparative mode for OPLC.
Have you ever found it hard to findcannabis documentaries cannabinoids facts or peer reviewed research to share with friends, family members, or associates? Well, look no further! Montana Biotech has been compiling the resources for you, the patient, provider, individual, or researcher. Feel free to share these links as you feel fit. You took the time to find these cannabis documentaries, cannabinoid facts, and marijuana peer reviewed research links here, no need to look further. As new content is found and shared, Montana Biotech will find the time to share.
Montana BioTech currently offers hermaphroditetesting and sexing of Cannabis 1-2 weeks after germination! So, if you are looking for the FASTEST method to sex your medical marijuana / cannabis seedlings, call on Montana Biotech! Male / Female Screening, Genetic Profiling:
Have you ever wanted to test your cannabisplant to see if it is a hermaphrodite? Well, Montana Biotech has a test for that! A cannabisplant will express hermaphroditic tendencies from environmental stresses or because of a genetic predisposition to express monoecious traits. A monoecious cannabisplant is a TRUE hermaphrodite. This cannabisplant will express male and female flower structures on the same plant. This is the hermaphrodite to be aware of! A true hermaphrodite sexes as a female early in the flowering cycle. A true hermaphrodite is a ticking time bomb! She/it will seed everything in your flowering room! Environmentally induced hermaphroditecannabisplants can not be tested for, at this time, and do not pose the same risk to the rest of the room. These hermaphrodites are not “true” hermaphrodites and usually pollinate themselves. ANY cannabisplant can be induced to revert to a hermaphroditic state by stress. By stressing a cannabisplant, you have tapped into its inherent survival programming. Our motto is, “Know before they show…”
Montana Biotech now offers a Polymerase Chain Reaction (PCR) based test for hermaphrodite screening of cannabisplants. KNOW if your cannabis plant has the TRUE hermaphrodite trait before you flower it!
Hermaphroditism in the cannabis sub species Indica, Sativa, or Ruderalis can be defined in terms of two types, a True Hermaphrodite or an Environmentally Induced Hermaphrodite. I will try to define the subtle differences. Hermaphroditism in cannabis is a genetically influenced trait. Both true and environmentally induced hermaphrodites can be genotypically and phenotypically observed.
A genotypic difference that can be observed between the two is the presence of both sex markers in true hermaphrodites. True hermaphrodites posses BOTH sex markers when analyzed using PCR techniques.
A phenotypic difference is the appearance of fully defined male flowers at the beginning of flower production verses the appearance of a male stamen in a female pistil dominate flower after flower production had been initiated. A True hermaphrodite has both fully formed male and female sex morphology at the same time of flower induction. Photos included below.
True Hermaphrodite in Cannabis
Environmentally Induced Hermaphrodite in Cannabis
Polymerase Chain Reaction (PCR) techniques can be employed to determine the type of hermaphroditic tendency a cannabis seedling posses one week after germination. The genotypic difference of hermaphroditism is present by the time the first true leaf emerges after the cotyledons appearance.
!!!Montana Biotech is NOW ACCEPTING PATIENTS!!!
If you have a qualifying medical condition, but cant afford the cost of MMJ registration, we may be able to help.
Wholesale prices are available for all of our products including a 90 day buy back guarantee. Please feel free to contact us 24/7 with any questions.
Have you ever found it hard to findcannabis documentaries cannabinoids facts or peer reviewed research to share with friends, family members, or associates? Well, look no further! Montana BioTech has been compiling the resources for you, the patient, provider, individual, or researcher. Feel free to share these links as you feel fit. You took the time to find these cannabis documentaries, cannabinoid facts, and marijuana peer reviewed research links here, no need to look further. As new content is found and shared, Montana Biotech will find the time to share.
Montana’s medical marijuana community has seen a huge shake up since July 1st of this year. Labs have gone dark do to the legislative pressures to “repeal” the will of the people. In true Montana style “patients” and “caregivers” banded together to halt the advancing armies of cannabis prohibition.
The Montana State Legislature passed the repeal in disguise Senate Bill 423. This NEW law reversed many of the advances we made, here in Montana, towards patient access to quality controlled medicinal grade cannabis and cannabis infused products.
Montana Biotech forged ahead in the less than conducive environment to maintain our doors being open to those interested in potencytesting their medical cannabis and cannabis isolates.
Check out our products, services and botanicalresearch at Montana Biotech:
Montana Biotech is here to HELP all those ready to take their cannabis to that next level of perfection! Our laboratory is accepting patients for research projects concerning cannabinoid delivery and efficacy.
*Need assistance in finding a doctor for a medical marijuana recommendation?
*Help setting up a lab quality grow operation?
*Ever wanted a hand with drying, curing, or infusing cannabis right?
*Want to know what cannabinoids are present in your medicine?
*Want to know the potency, how much THC, CBD is in your smokables, or isolates?
When cannabis is added to chloroform, for instance, it no longer qualifies as usable marijuana. No person can use the medical marijuana once it is added to an adulterant. To make analysis possible, a patient or provider would weigh 1 gram of cannabis and add it to the NEW Montana Biotech medical marijuana sample collection method. If concern over sample collection continues, contact Montana Biotech for other options that are available. If you are a patient providing for yourself, SB423 does not effect you submitting samples in anyway! Samples will be prepared in a way that the “useable medical marijuana” is rendered useless due to the addition of adulterants so human consumption is impossible. MontanaBiotech is a botanicalresearchlab located in Bozeman RE-discovering the science behind cannabis, from their genes to the final product.
The enzymes that synthesize and degrade the endocannabinoids. Unlike traditional neurotransmitters, endogenous cannabinoids are not stored in vesicles after synthesis, but are synthesized on demand (Rodriguez de Fonseca et al., 2004).[citation needed] However, some evidence suggests that a pool of synthesized endocannabinoids (namely, 2-AG) may exist without the requirement of on-demand synthesis.[1]
The endocannabinoid system has been studied using genetic and pharmacological methods. These studies have revealed a broad role for endocannabinoid signaling in a variety of physiological processes, including neuromodulator release,[2][3][4]motor learning,[5]synaptic plasticity,[6]appetite,[7] and pain sensation.[8]
In today’s environment of high competition in the medical marijuana industry, a way to distinguish what you have from your competitors is essential! This kit can be used to create a unique genetic profile of your hybrid! This PCR, gel electrophoresis based test can provide you a “picture” of YOUR plants genetic make-up! With this info in hand, it is easier to obtain a trademark on your variety / strain with the U.S. Patent and Trademark Office.
Another advantage that can be gained with the Plant DNA Collection Kit™ is TIME! TIME from seed, to clone, to harvest! At some point we all need to start from seed, whether it is starting a new variety / strain, or creating your own hybrid. It would be great to be able to weed the males from the females to increase productivity! This can be obtained with male / female sexing! Male / female sexing is a PCR based test. 1-2 weeks after germination of the seed, KNOW if it’s male or female by its GENES. NO GUESS WORK. By time that seedling is ready for cloning, KNOW that it is a FEMALE, and move on!! Maintain the phenotype without having to re-vegetate the plant, our having unwanted clones reducing cost!
Have you ever wanted to test your cannabis plant to see if it is a hermaphrodite? Well, Montana Biotech has a test for that! A cannabis plant will express hermaphroditic tendencies from environmental stresses or because of a genetic predisposition to express monoecious traits. A monoecious cannabis plant is a TRUE hermaphrodite. This cannabis plant will express male and female flower structures on the same plant. This is the hermaphrodite to be aware of! A true hermaphrodite sexes as a female early in the flowering cycle. A true hermaphrodite is a ticking time bomb! She/it will seed everything in your flowering room! Environmentally induced hermaphrodite cannabis plants can not be tested for, at this time, and do not pose the same risk to the rest of the room. These hermaphrodites are not “true” hermaphrodites and usually pollinate themselves. ANY cannabis plant can be induced to revert to a hermaphroditic state by stress. By stressing a cannabis plant, you have tapped into its inherent survival programming. Montana Biotech now offers a Polymerase Chain Reaction (PCR) based test for hermaphrodite screening of cannabis plants. KNOW if your cannabis plant has the TRUE hermaphrodite trait before you flower it! Our motto is, “Know before they show…”
Montana BioTech 