Qualitative v. Quantitative Testing Methods, Grow Buddy v. Cannalytics TLC Cannabinoid Analysis Technique and the Use of an Internal Standard

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QUALTITATIVE vs. QAUNTITATIVE Analysis of Cannabis THC, CBD, CBN Cannabinoid Levels

What does this have to do with medical marijuana cannabinoid quantification? Well, it matters when comparing AlphaNova’s Cannalytics TLC cannabinoid test kit verses Montana Biotech’s Grow Buddy THC, CBD, CBN, cannabinoid analysis kit.

To make this as clear as I can, Cannalytics is sold by Alpha Nova as a QAUNTITATIVE marijuana cannabinoid analysis testing kit using thin layer chromatography. On the other hand, Montana Biotech markets its T.L.C. THC, CBD, CBN analysis test kit as QUALTITATIVE.

AlphaNova’s MARKETS its Cannalytics TLC cannabinoid test kit as a QUALITATIVE test kit. This is FALSE!!! Thin Layer Chromatography is NOT a QUANTITATIVE, but a QUALITATIVE method for determining relative cannabinoid LEVELS. To sell or market a TLC kit as anything more than a qualitative test is FALSE ADVERTISING!! It is a LIE!!

“…We are proud to be able to now offer the updated and improved Cannalytics mini lab…”

“..5. The laminated photocopy sheet for measuring THC%, has been replaced by two transparencies, to allow for more precise

This is directly from their site, THC%, and is completely a FALSE statement!!!

Qualitative Analysis

Qualitative research is a method of inquiry employed in many different academic disciplines, traditionally in the social sciences, but also in market research and further contexts.[1] Qualitative researchers aim to gather an in-depth understanding of human behavior and the reasons that govern such behavior. The qualitative method investigates the why and how of decision making, not just what, where, when. Hence, smaller but focused samples are more often needed, rather than large samples.

Qualitative methods produce information only on the particular cases studied, and any more general conclusions are only propositions (informed assertions). Quantitative methods can be used to seek empirical support for such research hypotheses.

Qualitative analysis, quality. Grow Buddy TLC Kit.

Quantitative Analysis

Quantitative analysis, in chemistry, is the determination of the absolute or relative abundance (often expressed as a concentration) of one, several or all particular substance(s) present in a sample.

Once the presence of certain substance(s) in a sample is known, the study of their absolute or relative abundance can help in determining specific properties. Knowing the composition of a sample is very important and several ways have been developed to make it possible, like gravimetric and volumetric analysis. Gravimetric analysis yields more accurate data about the composition of a sample than volumetric analysis does, but the first one takes more time to perform in the laboratory. Volumetric analysis in the other side doesn’t take that much time and the results that we obtain are in the most cases satisfactory. Volumetric analysis can be simply a titration based in a neutralization reaction but it can also be a precipitation or a complex[disambiguation needed] forming reaction as well as a titration based in an redox reaction. However, each method in quantitative analysis has a general specification, in neutralization reactions, for example, the reaction that occurs is between an acid and a base, which yields a salt and water, hence the name neutralization. In the precipitation reactions the standard solution is in the most cases silver nitrate which is used as a reagent to react with the ions present in the sample and to form a high insoluble precipitate. Precipitation methods are often called simply as Argentometry. In the two other methods the situation is the same. Complex forming titration is a reaction that occurs between metal ions and a standard solution that is in the most cases EDTA (ethylene diamine tetra acetic acid). In the redox titration that reaction is carried out between an oxidizing agent and a reduction agent.

For example, quantitative analysis performed by mass spectrometry on biological samples can determine, by the relative abundance ratio of specific proteins, indications of certain diseases, like cancer.

Quantitative analysis, quantity. Cannalytics TLC Kit.

Quantitative vs. Qualitative Analysis

The general expression Qualitative Analysis […] refers to analyses in which substances are identified or classified on the basis of their chemical or physical properties, such as chemical reactivity, solubility, molecular weight, melting point, radiative properties (emission, absorption), mass spectra, nuclear half-life, etc. Quantitative Analysis refers to analyses in which the amount or concentration of an analyte may be determined (estimated) and expressed as a numerical value in appropriate units. Qualitative Analysis may take place without Quantitative Analysis, but Quantitative Analysis requires the identification (qualification) of the analyte for which numerical estimates are given.

International Union of Pure and Applied Chemistry, Nomenclature in evaluation of analytical methods including detection and quantification capabilities, Pure & Appl. Chem. 67(10), p. 1701 (1995)

Internal Standard

An internal standard in analytical chemistry is a chemical substance that is added in a constant amount to samples, the blank and calibration standards in a chemical analysis. This substance can then be used for calibration by plotting the ratio of the analyte signal to the internal standard signal as a function of the analyte concentration of the standards. This is done to correct for the loss of analyte during sample preparation or sample inlet. The internal standard is a compound that is very similar, but not identical to the chemical species of interest in the samples, as the effects of sample preparation should, relative to the amount of each species, be the same for the signal from the internal standard as for the signal(s) from the species of interest in the ideal case. Adding known quantities of analyte(s) of interest is a distinct technique called standard addition, which is performed to correct for matrix effects.

This ratio for the samples is then used to obtain their analyte concentrations from a calibration curve. The internal standard used needs to provide a signal that is similar to the analyte signal in most ways but sufficiently different so that the two signals are readily distinguishable by the instrument. For example deuterated chlorobenzene (C6D5Cl) is an internal standard used in the analysis of volatiles on GC-MS because it is similar to Chlorobenzene but does not occur naturally. Nor-Leucine is also a popular internal standard for the analysis of amino acids via GC-MS.

In NMR spectroscopy of the nuclei 1H, 13C and 29Si, frequencies depend on the magnetic field, which is not the same across all experiments. Therefore, frequencies are reported as relative differences to the internal standard tetramethylsilane. This relative difference to TMS is called chemical shift, and measured in parts per million.

In practice, since the difference between the deuterium signals of the solvent and TMS are known, no TMS may need to be added. By specifying the lock solvent to be used, modern spectrometers are able to correctly reference the sample; in effect, the deuterated solvent serves as the internal standard.

Definitions Care of Wikipedia

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According to the UNODC and the NIDA, TLC, as a method, is NOT accurate for the determination of relative or absolute LEVELS of cannabinoids in cannabis hemp or marijuana! To gain a more quantitative determination of cannabinoids in a sample, an internal standard will need to be analyzed with the sample in question. If an internal standard is used, a more accurate quantitative analysis can be made using thin layer chromatography.

To get TRUE quantitative analysis, a Gas Chromatograph (G.C) or High Pressure Liquid Chromatograph (H.P.L.C) should be used to quantify THC, CBD, CBN levels in a medical marijuana sample.


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